En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles

S. Hara-Kuge, Osamu Kuge, L. Orci, M. Amherdt, M. Ravazzola, F. T. Wieland, J. E. Rothman

Research output: Contribution to journalArticle

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Abstract

The cDNA encoding ε-COP, the 36-kD subunit of coatomer, was cloned from a bovine liver cDNA library and sequenced. Immunoblotting with an anti-ε-COP antibody showed that ε-COP exists in COP-coated vesicles as well as in the cytosolic coatomer. Using the cloned cDNA, recombinant His6-tagged ε-COP was overexpressed in cultured Chinese hamster ovary (CHO) cells, from which metabolically radiolabeled coatomer was purified by taking advantage of the His6 tag. Radiolabeled coatomer was employed to establish that all the subunits of the coatomer enter coated vesicles as an intact unit.

Original languageEnglish
Pages (from-to)883-892
Number of pages10
JournalJournal of Cell Biology
Volume124
Issue number6
Publication statusPublished - Jan 1 1994
Externally publishedYes

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COP-Coated Vesicles
His-His-His-His-His-His
Complementary DNA
Cricetulus
Gene Library
Immunoblotting
Anti-Idiotypic Antibodies
Ovary
Liver

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Hara-Kuge, S., Kuge, O., Orci, L., Amherdt, M., Ravazzola, M., Wieland, F. T., & Rothman, J. E. (1994). En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles. Journal of Cell Biology, 124(6), 883-892.

En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles. / Hara-Kuge, S.; Kuge, Osamu; Orci, L.; Amherdt, M.; Ravazzola, M.; Wieland, F. T.; Rothman, J. E.

In: Journal of Cell Biology, Vol. 124, No. 6, 01.01.1994, p. 883-892.

Research output: Contribution to journalArticle

Hara-Kuge, S, Kuge, O, Orci, L, Amherdt, M, Ravazzola, M, Wieland, FT & Rothman, JE 1994, 'En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles', Journal of Cell Biology, vol. 124, no. 6, pp. 883-892.
Hara-Kuge S, Kuge O, Orci L, Amherdt M, Ravazzola M, Wieland FT et al. En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles. Journal of Cell Biology. 1994 Jan 1;124(6):883-892.
Hara-Kuge, S. ; Kuge, Osamu ; Orci, L. ; Amherdt, M. ; Ravazzola, M. ; Wieland, F. T. ; Rothman, J. E. / En bloc incorporation of coatomer subunits during the assembly of COP- coated vesicles. In: Journal of Cell Biology. 1994 ; Vol. 124, No. 6. pp. 883-892.
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