Enantioselective recognition mechanism of secondary alcohol by surfactant-coated lipases in nonaqueous media

Noriho Kamiya, Hideaki Kasagi, Masahito Inoue, Koichiro Kusunoki, Masahiro Goto

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The enantioselective recognition mechanism of secondary alcohol by lipases originated from Candida rugosa and Pseudomonas cepacia was elucidated on the basis of the kinetic study of the esterification of alcohol with lauric acid in isooctane. To obtain inherent kinetic parameters, we utilized a surfactant-coated lipase whose conformation is considered to be an 'open' form in a homogeneous organic solvent. Based on the experimental results, the enantioselectivity of lipases was found to be derived from the difference in the V(max) values between the two enantiomers. The same result was observed when lipases of different origin and substrates with different molecular structures were applied.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalBiotechnology and Bioengineering
Volume65
Issue number2
DOIs
Publication statusPublished - Oct 20 1999

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Lipases
Lipase
Surface-Active Agents
Alcohols
Surface active agents
lauric acid
Burkholderia cepacia
Enantiomers
Candida
Esterification
Enantioselectivity
Molecular Structure
Kinetic parameters
Organic solvents
Molecular structure
Conformations
Kinetics
Acids
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Enantioselective recognition mechanism of secondary alcohol by surfactant-coated lipases in nonaqueous media. / Kamiya, Noriho; Kasagi, Hideaki; Inoue, Masahito; Kusunoki, Koichiro; Goto, Masahiro.

In: Biotechnology and Bioengineering, Vol. 65, No. 2, 20.10.1999, p. 227-232.

Research output: Contribution to journalArticle

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