TY - JOUR
T1 - Enantioselective visualization of D-alanine in rat anterior pituitary gland
T2 - Localization to ACTH-secreting cells
AU - Etoh, Sachise
AU - Hamase, Kenji
AU - Morikawa, Akiko
AU - Ohgusu, Tomohiro
AU - Zaitsu, Kiyoshi
N1 - Funding Information:
Acknowledgments This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and Industrial Technology Research Grant Program in ‘05 from New Energy and Industrial Technology Development Organization (NEDO) of Japan.
PY - 2009/1
Y1 - 2009/1
N2 - The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.
AB - The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.
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U2 - 10.1007/s00216-008-2401-5
DO - 10.1007/s00216-008-2401-5
M3 - Article
C2 - 18839155
AN - SCOPUS:57849095691
SN - 0016-1152
VL - 393
SP - 217
EP - 223
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
IS - 1
ER -