Enantioselective visualization of D-alanine in rat anterior pituitary gland

Localization to ACTH-secreting cells

Sachise Etoh, Kenji Hamase, Akiko Morikawa, Tomohiro Ohgusu, Kiyoshi Zaitsu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume393
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

Anterior Pituitary Gland
Alanine
Adrenocorticotropic Hormone
Rats
Visualization
Prolactin
Blood Glucose
Hormones
Insulin
D-Aspartic Acid
Mammals
Thyrotropin
Staining and Labeling
Growth Hormone
Insulin-Secreting Cells
Pituitary Gland
Monoclonal Antibodies
Tissue
Pancreas

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Enantioselective visualization of D-alanine in rat anterior pituitary gland : Localization to ACTH-secreting cells. / Etoh, Sachise; Hamase, Kenji; Morikawa, Akiko; Ohgusu, Tomohiro; Zaitsu, Kiyoshi.

In: Analytical and Bioanalytical Chemistry, Vol. 393, No. 1, 01.01.2009, p. 217-223.

Research output: Contribution to journalArticle

Etoh, Sachise ; Hamase, Kenji ; Morikawa, Akiko ; Ohgusu, Tomohiro ; Zaitsu, Kiyoshi. / Enantioselective visualization of D-alanine in rat anterior pituitary gland : Localization to ACTH-secreting cells. In: Analytical and Bioanalytical Chemistry. 2009 ; Vol. 393, No. 1. pp. 217-223.
@article{7f3c19fdfd7f4e6d99f882644255ef86,
title = "Enantioselective visualization of D-alanine in rat anterior pituitary gland: Localization to ACTH-secreting cells",
abstract = "The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.",
author = "Sachise Etoh and Kenji Hamase and Akiko Morikawa and Tomohiro Ohgusu and Kiyoshi Zaitsu",
year = "2009",
month = "1",
day = "1",
doi = "10.1007/s00216-008-2401-5",
language = "English",
volume = "393",
pages = "217--223",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Enantioselective visualization of D-alanine in rat anterior pituitary gland

T2 - Localization to ACTH-secreting cells

AU - Etoh, Sachise

AU - Hamase, Kenji

AU - Morikawa, Akiko

AU - Ohgusu, Tomohiro

AU - Zaitsu, Kiyoshi

PY - 2009/1/1

Y1 - 2009/1/1

N2 - The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.

AB - The cellular localization of D-alanine (D-Ala) in the rat pituitary gland, the tissue containing the highest amount of D-Ala, has been clarified for the first time by enantioselective visualization of D-Ala using our own established mouse monoclonal antibody against D-Ala. D-Ala immunopositive cells were present predominantly in the anterior lobe, while no intense staining was observed in the intermediate and posterior lobes. The anterior pituitary gland contains five types of cells secreting specific hormones (growth hormone, adrenocorticotropic hormone (ACTH), gonadotropic hormone, prolactin, and thyroid-stimulating hormone), and the double staining results indicated that D-Ala is localized to the ACTH-secreting cells. The localization of D-Ala is clearly different from that of D-aspartic acid (D-Asp), which is observed in the prolactin cells. Considered together with our previous findings that D-Ala is localized to the insulin-secreting beta-cells in the pancreas, and both ACTH and insulin are typical regulatory hormones of blood glucose, D-Ala is suggested to have some functional relationships to blood glucose level regulation in mammals.

UR - http://www.scopus.com/inward/record.url?scp=57849095691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57849095691&partnerID=8YFLogxK

U2 - 10.1007/s00216-008-2401-5

DO - 10.1007/s00216-008-2401-5

M3 - Article

VL - 393

SP - 217

EP - 223

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 1

ER -