Encephalomyocarditis Virus Viroporin 2B Activates NLRP3 Inflammasome

Minako Ito, Yusuke Yanagi, Takeshi Ichinohe

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Nod-like receptors (NLRs) comprise a large family of intracellular pattern- recognition receptors. Members of the NLR family assemble into large multiprotein complexes, termed the inflammasomes. The NLR family, pyrin domain-containing 3 (NLRP3) is triggered by a diverse set of molecules and signals, and forms the NLRP3 inflammasome. Recent studies have indicated that both DNA and RNA viruses stimulate the NLRP3 inflammasome, leading to the secretion of interleukin 1 beta (IL-1β) and IL-18 following the activation of caspase-1. We previously demonstrated that the proton-selective ion channel M2 protein of influenza virus activates the NLRP3 inflammasome. However, the precise mechanism by which NLRP3 recognizes viral infections remains to be defined. Here, we demonstrate that encephalomyocarditis virus (EMCV), a positive strand RNA virus of the family Picornaviridae, activates the NLRP3 inflammasome in mouse dendritic cells and macrophages. Although transfection with RNA from EMCV virions or EMCV-infected cells induced robust expression of type I interferons in macrophages, it failed to stimulate secretion of IL-1β. Instead, the EMCV viroporin 2B was sufficient to cause inflammasome activation in lipopolysaccharide-primed macrophages. While cells untransfected or transfected with the gene encoding the EMCV non-structural protein 2A or 2C expressed NLRP3 uniformly throughout the cytoplasm, NLRP3 was redistributed to the perinuclear space in cells transfected with the gene encoding the EMCV 2B or influenza virus M2 protein. 2B proteins of other picornaviruses, poliovirus and enterovirus 71, also caused the NLRP3 redistribution. Elevation of the intracellular Ca2+ level, but not mitochondrial reactive oxygen species and lysosomal cathepsin B, was important in EMCV-induced NLRP3 inflammasome activation. Chelation of extracellular Ca2+ did not reduce virus-induced IL-1β secretion. These results indicate that EMCV activates the NLRP3 inflammasome by stimulating Ca2+ flux from intracellular storages to the cytosol, and highlight the importance of viroporins, transmembrane pore-forming viral proteins, in virus-induced NLRP3 inflammasome activation.

Original languageEnglish
Article numbere1002857
JournalPLoS pathogens
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 1 2012

Fingerprint

Encephalomyocarditis virus
Inflammasomes
RNA Viruses
Interleukin-1beta
Picornaviridae
Macrophages
Orthomyxoviridae
Viruses
Viral Structural Proteins
Pattern Recognition Receptors
Multiprotein Complexes
Caspase 1
Cathepsin B
Interferon Type I
Proteins
Interleukin-18
DNA Viruses
Poliovirus
Enterovirus
Viral Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Encephalomyocarditis Virus Viroporin 2B Activates NLRP3 Inflammasome. / Ito, Minako; Yanagi, Yusuke; Ichinohe, Takeshi.

In: PLoS pathogens, Vol. 8, No. 8, e1002857, 01.08.2012.

Research output: Contribution to journalArticle

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