Endocardial versus epicardial differences of sarcoplasmic reticulum Ca2+-ATPase gene expression in the canine failing myocardium

K. Igarashi-Saito, H. Tsutsui, M. Takahashi, S. Kinugawa, K. Egashira, A. Takeshita

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It is unknown whether the transmural heterogeneity of sarcoplasmic reticulum (SR) Ca2+-ATPase gene expression is present within the left ventricular (LV) wall. Moreover, the changes of transmural distribution have not been examined in the failing hearts. We thus quantified steady-state mRNA abundance of SR Ca2+ regulatory proteins by Northern blot analysis in both subendocardial and subepicardial LV layers from normal and rapid pacing-induced heart failure (HF) dog hearts. For normal LV, Ca2+-ATPase mRNA abundance (normalized to glyceraldehyde-3-phosphate dehydrogenase [GAPDH] mRNA) was significantly reduced in the subendocardium, whereas calsequestrin mRNA abundance was comparable between the two layers. For HF LV, Ca2+-ATPase mRNA abundance in the subendocardium was also reduced compared to the subepicardium. However, the endocardium to epicardium ratio was comparable between control and HF (0.62 ± 0.08 vs. 0.65 ± 0.07; p = NS). Therefore, the transmural gradient of this gene was constant in both control and HF. Even though the data on the transmural heterogeneity of protein level is not available, the subendocardium contained significantly less Ca2+-ATPase mRNA, which might contribute, at least in part, to the transmural gradients of biochemical and mechanical function.

Original languageEnglish
Pages (from-to)267-273
Number of pages7
JournalBasic Research in Cardiology
Volume94
Issue number4
DOIs
Publication statusPublished - Aug 1 1999

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Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Canidae
Myocardium
Gene Expression
Messenger RNA
Heart Failure
Calsequestrin
Endocardium
Glyceraldehyde-3-Phosphate Dehydrogenases
Pericardium
Northern Blotting
Proteins
Dogs
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Endocardial versus epicardial differences of sarcoplasmic reticulum Ca2+-ATPase gene expression in the canine failing myocardium. / Igarashi-Saito, K.; Tsutsui, H.; Takahashi, M.; Kinugawa, S.; Egashira, K.; Takeshita, A.

In: Basic Research in Cardiology, Vol. 94, No. 4, 01.08.1999, p. 267-273.

Research output: Contribution to journalArticle

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