Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA

Tatsuro Yamamoto, Chiyomi Sakamoto, Hiroaki Tachiwana, Mitsuru Kumabe, Toshiro Matsui, Tadatoshi Yamashita, Masatoshi Shinagawa, Koji Ochiai, Noriko Saitoh, Mitsuyoshi Nakao

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Abstract

Long-term estrogen deprivation (LTED) of an estrogen receptor (ER) α-positive breast cancer cell line recapitulates cancer cells that have acquired estrogen-independent cell proliferation and endocrine therapy resistance. Previously, we have shown that a cluster of non-coding RNAs, Eleanors (ESR1 locus enhancing and activating non-coding RNAs) formed RNA cloud and upregulated the ESR1 gene in the nuclei of LTED cells. Eleanors were inhibited by resveratrol through ER. Here we prepared another polyphenol, glyceollin I from stressed soybeans, and identified it as a major inhibitor of the Eleanor RNA cloud and ESR1 mRNA transcription. The inhibition was independent of ER, unlike one by resveratrol. This was consistent with a distinct tertiary structure of glyceollin I for ER binding. Glyceollin I preferentially inhibited the growth of LTED cells and induced apoptosis. Our results suggest that glyceollin I has a novel role in LTED cell inhibition through Eleanors. In other words, LTED cells or endocrine therapy-resistant breast cancer cells may be ready for apoptosis, which can be triggered with polyphenols both in ER-dependent and ER-independent manners.

Original languageEnglish
Article number15202
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

All Science Journal Classification (ASJC) codes

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    Yamamoto, T., Sakamoto, C., Tachiwana, H., Kumabe, M., Matsui, T., Yamashita, T., Shinagawa, M., Ochiai, K., Saitoh, N., & Nakao, M. (2018). Endocrine therapy-resistant breast cancer model cells are inhibited by soybean glyceollin I through Eleanor non-coding RNA. Scientific reports, 8(1), [15202]. https://doi.org/10.1038/s41598-018-33227-y