Endogenous glucocorticoids inhibit scratching behavior induced by the administration of compound 48/80 in mice

Kenji Hirayama, Nobuyuki Sudo, Masanori Sueyasu, Junko Sonoda, Youich Chida, Ryozo Oishi, Chiharu Kubo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we investigated the effects of endogenous glucocorticoids on the compound 48/80 (a condensation product of N-methyl-p- methoxyphenethylamine with formaldehyde)-induced mouse scratching behavior using either RU-486 (mifepristone), a glucocorticoid receptor antagonist, or a surgical resection of the adrenal glands. Subcutaneous injection of compound 48/80 induced not only a corticosterone elevation in the plasma but also an enhanced expression of corticotropin releasing hormone (CRH) mRNA in the paraventricular nucleus, which thus suggests that hypothalamic-pituitary- adrenal axis is activated by the compound 48/80-induced cutaneous reaction. Inhibition of such an endogenous glucocorticoid activity by RU-486 significantly increased the degree of scratching behavior at not only the early-phase (<60 min) but also the late-phase (>60 min) time course after the injection of compound 48/80. Since the elevation of the histamine levels in the plasma in the RU-486-treated mice was no longer found in late-phase scratching behavior, these results thus indicate that histamine is a dominant mediator responsible for early-phase scratching behavior, while different mediators other than histamine may be also involved in the induction of late-phase scratching behavior. Moreover, surgical removal of adrenal glands also significantly increased the compound 48/80-induced scratching behavior without affecting anxiety and locomotor parameters, indicating that endogenous glucocorticoids exert their anti-pururitogenic effects independently of changes in behavioral performance. In conclusion, endogenous glucocorticoid activity was found to suppress the compound 48/80-induced scratching behavior in mice.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalEuropean Journal of Pharmacology
Volume481
Issue number1
DOIs
Publication statusPublished - Nov 14 2003

Fingerprint

p-Methoxy-N-methylphenethylamine
Glucocorticoids
Mifepristone
Histamine
Adrenal Glands
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Glucocorticoid Receptors
Subcutaneous Injections
Corticosterone
Formaldehyde
Anxiety
Messenger RNA
Skin
Injections

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Endogenous glucocorticoids inhibit scratching behavior induced by the administration of compound 48/80 in mice. / Hirayama, Kenji; Sudo, Nobuyuki; Sueyasu, Masanori; Sonoda, Junko; Chida, Youich; Oishi, Ryozo; Kubo, Chiharu.

In: European Journal of Pharmacology, Vol. 481, No. 1, 14.11.2003, p. 59-65.

Research output: Contribution to journalArticle

Hirayama, Kenji ; Sudo, Nobuyuki ; Sueyasu, Masanori ; Sonoda, Junko ; Chida, Youich ; Oishi, Ryozo ; Kubo, Chiharu. / Endogenous glucocorticoids inhibit scratching behavior induced by the administration of compound 48/80 in mice. In: European Journal of Pharmacology. 2003 ; Vol. 481, No. 1. pp. 59-65.
@article{1bff3fd3f754415887e1445198247ee3,
title = "Endogenous glucocorticoids inhibit scratching behavior induced by the administration of compound 48/80 in mice",
abstract = "In this study, we investigated the effects of endogenous glucocorticoids on the compound 48/80 (a condensation product of N-methyl-p- methoxyphenethylamine with formaldehyde)-induced mouse scratching behavior using either RU-486 (mifepristone), a glucocorticoid receptor antagonist, or a surgical resection of the adrenal glands. Subcutaneous injection of compound 48/80 induced not only a corticosterone elevation in the plasma but also an enhanced expression of corticotropin releasing hormone (CRH) mRNA in the paraventricular nucleus, which thus suggests that hypothalamic-pituitary- adrenal axis is activated by the compound 48/80-induced cutaneous reaction. Inhibition of such an endogenous glucocorticoid activity by RU-486 significantly increased the degree of scratching behavior at not only the early-phase (<60 min) but also the late-phase (>60 min) time course after the injection of compound 48/80. Since the elevation of the histamine levels in the plasma in the RU-486-treated mice was no longer found in late-phase scratching behavior, these results thus indicate that histamine is a dominant mediator responsible for early-phase scratching behavior, while different mediators other than histamine may be also involved in the induction of late-phase scratching behavior. Moreover, surgical removal of adrenal glands also significantly increased the compound 48/80-induced scratching behavior without affecting anxiety and locomotor parameters, indicating that endogenous glucocorticoids exert their anti-pururitogenic effects independently of changes in behavioral performance. In conclusion, endogenous glucocorticoid activity was found to suppress the compound 48/80-induced scratching behavior in mice.",
author = "Kenji Hirayama and Nobuyuki Sudo and Masanori Sueyasu and Junko Sonoda and Youich Chida and Ryozo Oishi and Chiharu Kubo",
year = "2003",
month = "11",
day = "14",
doi = "10.1016/j.ejphar.2003.09.003",
language = "English",
volume = "481",
pages = "59--65",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Endogenous glucocorticoids inhibit scratching behavior induced by the administration of compound 48/80 in mice

AU - Hirayama, Kenji

AU - Sudo, Nobuyuki

AU - Sueyasu, Masanori

AU - Sonoda, Junko

AU - Chida, Youich

AU - Oishi, Ryozo

AU - Kubo, Chiharu

PY - 2003/11/14

Y1 - 2003/11/14

N2 - In this study, we investigated the effects of endogenous glucocorticoids on the compound 48/80 (a condensation product of N-methyl-p- methoxyphenethylamine with formaldehyde)-induced mouse scratching behavior using either RU-486 (mifepristone), a glucocorticoid receptor antagonist, or a surgical resection of the adrenal glands. Subcutaneous injection of compound 48/80 induced not only a corticosterone elevation in the plasma but also an enhanced expression of corticotropin releasing hormone (CRH) mRNA in the paraventricular nucleus, which thus suggests that hypothalamic-pituitary- adrenal axis is activated by the compound 48/80-induced cutaneous reaction. Inhibition of such an endogenous glucocorticoid activity by RU-486 significantly increased the degree of scratching behavior at not only the early-phase (<60 min) but also the late-phase (>60 min) time course after the injection of compound 48/80. Since the elevation of the histamine levels in the plasma in the RU-486-treated mice was no longer found in late-phase scratching behavior, these results thus indicate that histamine is a dominant mediator responsible for early-phase scratching behavior, while different mediators other than histamine may be also involved in the induction of late-phase scratching behavior. Moreover, surgical removal of adrenal glands also significantly increased the compound 48/80-induced scratching behavior without affecting anxiety and locomotor parameters, indicating that endogenous glucocorticoids exert their anti-pururitogenic effects independently of changes in behavioral performance. In conclusion, endogenous glucocorticoid activity was found to suppress the compound 48/80-induced scratching behavior in mice.

AB - In this study, we investigated the effects of endogenous glucocorticoids on the compound 48/80 (a condensation product of N-methyl-p- methoxyphenethylamine with formaldehyde)-induced mouse scratching behavior using either RU-486 (mifepristone), a glucocorticoid receptor antagonist, or a surgical resection of the adrenal glands. Subcutaneous injection of compound 48/80 induced not only a corticosterone elevation in the plasma but also an enhanced expression of corticotropin releasing hormone (CRH) mRNA in the paraventricular nucleus, which thus suggests that hypothalamic-pituitary- adrenal axis is activated by the compound 48/80-induced cutaneous reaction. Inhibition of such an endogenous glucocorticoid activity by RU-486 significantly increased the degree of scratching behavior at not only the early-phase (<60 min) but also the late-phase (>60 min) time course after the injection of compound 48/80. Since the elevation of the histamine levels in the plasma in the RU-486-treated mice was no longer found in late-phase scratching behavior, these results thus indicate that histamine is a dominant mediator responsible for early-phase scratching behavior, while different mediators other than histamine may be also involved in the induction of late-phase scratching behavior. Moreover, surgical removal of adrenal glands also significantly increased the compound 48/80-induced scratching behavior without affecting anxiety and locomotor parameters, indicating that endogenous glucocorticoids exert their anti-pururitogenic effects independently of changes in behavioral performance. In conclusion, endogenous glucocorticoid activity was found to suppress the compound 48/80-induced scratching behavior in mice.

UR - http://www.scopus.com/inward/record.url?scp=0344153889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344153889&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2003.09.003

DO - 10.1016/j.ejphar.2003.09.003

M3 - Article

C2 - 14637175

AN - SCOPUS:0344153889

VL - 481

SP - 59

EP - 65

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -