Endogenous interleukin 10 prevents apoptosis in macrophages during salmonella infection

Toshiyuki Arai, Kenji Hiromatsu, Hitoshi Nishimura, Yuki Kimura, Noritada Kobayashi, Hiroshi Ishida, Yuji Nimura, Yasunobu Yoshikai

    Research output: Contribution to journalArticlepeer-review

    64 Citations (Scopus)

    Abstract

    To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-α production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-α-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-α after killing of Salmonella.

    Original languageEnglish
    Pages (from-to)600-607
    Number of pages8
    JournalBiochemical and Biophysical Research Communications
    Volume213
    Issue number2
    DOIs
    Publication statusPublished - 1995

    All Science Journal Classification (ASJC) codes

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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