TY - JOUR
T1 - Endomucin is expressed in embryonic dorsal aorta and is able to inhibit cell adhesion
AU - Ueno, Masaya
AU - Igarashi, Katsuhide
AU - Kimura, Naoki
AU - Okita, Keisuke
AU - Takizawa, Makiko
AU - Nobuhisa, Ikuo
AU - Kojima, Tetsuo
AU - Kitamura, Toshio
AU - Samulowitz, Ulrike
AU - Vestweber, Dietmar
AU - Shimomura, Taizo
AU - Suda, Toshio
AU - Nakashima, Kinichi
AU - Taga, Tetsuya
N1 - Funding Information:
We are very much grateful to Ms. Yuki Noguchi for her excellent secretarial assistance. We also thank Ms. Kaori Kaneko for her technical help. This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Takeda Science Foundation, and Human Frontier Science Program.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001/9/21
Y1 - 2001/9/21
N2 - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
AB - Recent studies have suggested the existence of progenitors common to hematopoietic and endothelial cells, called hemangioblasts, in, for instance, embryonic dorsal aorta. To identify a membrane-bound or secretory molecule regulating early hematopoiesis, we screened a cDNA library from dorsal aortas of embryonic day (E) 10.5 mice by a signal sequence trap method and obtained a clone encoding a sialoprotein, endomucin-1. Immunohistochemistry revealed that the endomucin-1 transcript was specifically expressed in the endothelial cells of dorsal aorta of E10.5 mouse embryo. Overexpression of endomucin-1 strongly inhibited adhesion and aggregation of cells, including cultured endothelial cells from E10.5 dorsal aorta. These data suggest that endomucin-1 may play a role in detachment of hematopoietic cells from endothelium during early hematopoiesis.
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U2 - 10.1006/bbrc.2001.5587
DO - 10.1006/bbrc.2001.5587
M3 - Article
C2 - 11554756
AN - SCOPUS:0035929358
VL - 287
SP - 501
EP - 506
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -