TY - JOUR
T1 - EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus
AU - Ishino, Sonoko
AU - Makita, Naruto
AU - Shiraishi, Miyako
AU - Yamagami, Takeshi
AU - Ishino, Yoshizumi
N1 - Funding Information:
This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan [grant numbers 21113005 , 23310152 , and 26242075 to Y. I.]. M. S. was supported by a Grant-in-Aid for Japan Society for the Promotion of Science (JSPS) Fellows [grant number, 26-9354 ]. S. I. was partly supported by Institute for fermentation, Osaka (IFO).
PY - 2015/3/29
Y1 - 2015/3/29
N2 - Base deamination is a typical form of DNA damage, and it must be repaired quickly to maintain the genome integrity of living organisms. Endonuclease Q (EndoQ), recently found in the hyperthermophilic archaea, is an enzyme that cleaves the phosphodiester bond 5′ from the damaged nucleotide in the DNA strand, and may primarily function to start the repair process for the damaged bases. Endonuclease V (EndoV) also hydrolyzes the second phosphodiester bond 3′ from the damaged nucleotide, although the hyperthermophilic archaeal EndoV is a strictly hypoxanthine-specific endonuclease. To understand the relationships of the EndoQ and EndoV functions in hyperthermophilic archaea, we analyzed their interactions in hypoxanthine repair. EndoQ and EndoV do not directly interact with each other in either the presence or absence of DNA. However, EndoQ and EndoV individually worked on deoxyinosine (dI)-containing DNA at each cleavage site. EndoQ has higher affinity to dI-containing DNA than EndoV, and cells produce higher amounts of EndoQ, as compared to EndoV. These data support the proposal that EndoQ primarily functions for, at least, dI-containing DNA.
AB - Base deamination is a typical form of DNA damage, and it must be repaired quickly to maintain the genome integrity of living organisms. Endonuclease Q (EndoQ), recently found in the hyperthermophilic archaea, is an enzyme that cleaves the phosphodiester bond 5′ from the damaged nucleotide in the DNA strand, and may primarily function to start the repair process for the damaged bases. Endonuclease V (EndoV) also hydrolyzes the second phosphodiester bond 3′ from the damaged nucleotide, although the hyperthermophilic archaeal EndoV is a strictly hypoxanthine-specific endonuclease. To understand the relationships of the EndoQ and EndoV functions in hyperthermophilic archaea, we analyzed their interactions in hypoxanthine repair. EndoQ and EndoV do not directly interact with each other in either the presence or absence of DNA. However, EndoQ and EndoV individually worked on deoxyinosine (dI)-containing DNA at each cleavage site. EndoQ has higher affinity to dI-containing DNA than EndoV, and cells produce higher amounts of EndoQ, as compared to EndoV. These data support the proposal that EndoQ primarily functions for, at least, dI-containing DNA.
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U2 - 10.1016/j.biochi.2015.06.015
DO - 10.1016/j.biochi.2015.06.015
M3 - Article
C2 - 26116888
AN - SCOPUS:84946491092
VL - 118
SP - 264
EP - 269
JO - Biochimie
JF - Biochimie
SN - 0300-9084
ER -