Abstract
Cardiotrophin-1 (CT-1) a novel IL-6-related cytokine, induces distinct hypertrophic responses to endothelin-1 (ET-1) on cultured neonatal rat cardiac myocytes. We found that ET-1 and CT-1 show a distinct pattern of gene induction of natriuretic peptides. Elucidation of the transcriptional mechanisms of brain natriuretic peptide (BNP) gene induction by ET-1 or CT-1 will provide better information for our understanding of the molecular mechanisms of cardiac hypertrophy. In this study, reporter constructs containing the human BNP 5′ flanking sequence were transfected into neonatal rat cardiac myocytes and the cells were stimulated with ET-1 or CT-1. A total of 1813 bp of the human BNP 5′ flanking sequence conferred an ET-1 inducibility on the reporter gene. However, it did not confer CT-1 inducibility. These results show that distinct mechanisms are involved in BNP gene induction by ET-1 or CT-1, and (in this study) that the CT-1 responsive element is not located in the region examined.
| Original language | English |
|---|---|
| Journal | Journal of Cardiovascular Pharmacology |
| Volume | 31 |
| Issue number | SUPPL. 1 |
| DOIs | |
| Publication status | Published - Jan 1 1998 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Cardiology and Cardiovascular Medicine
Cite this
Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms. / Kuwahara, Koichiro; Saito, Yoshihiko; Ogawa, Yoshihiro; Tamura, Naohisa; Ishikawa, Masahiro; Harada, Masaki; Ogawa, Emiko; Miyamoto, Yoshihiro; Hamanaka, Ichiro; Kamitani, Shigeki; Kajiyama, Noboru; Takahashi, Nobuki; Nakagawa, Osamu; Masuda, Izuru; Nakao, Kazuwa.
In: Journal of Cardiovascular Pharmacology, Vol. 31, No. SUPPL. 1, 01.01.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms
AU - Kuwahara, Koichiro
AU - Saito, Yoshihiko
AU - Ogawa, Yoshihiro
AU - Tamura, Naohisa
AU - Ishikawa, Masahiro
AU - Harada, Masaki
AU - Ogawa, Emiko
AU - Miyamoto, Yoshihiro
AU - Hamanaka, Ichiro
AU - Kamitani, Shigeki
AU - Kajiyama, Noboru
AU - Takahashi, Nobuki
AU - Nakagawa, Osamu
AU - Masuda, Izuru
AU - Nakao, Kazuwa
PY - 1998/1/1
Y1 - 1998/1/1
N2 - Cardiotrophin-1 (CT-1) a novel IL-6-related cytokine, induces distinct hypertrophic responses to endothelin-1 (ET-1) on cultured neonatal rat cardiac myocytes. We found that ET-1 and CT-1 show a distinct pattern of gene induction of natriuretic peptides. Elucidation of the transcriptional mechanisms of brain natriuretic peptide (BNP) gene induction by ET-1 or CT-1 will provide better information for our understanding of the molecular mechanisms of cardiac hypertrophy. In this study, reporter constructs containing the human BNP 5′ flanking sequence were transfected into neonatal rat cardiac myocytes and the cells were stimulated with ET-1 or CT-1. A total of 1813 bp of the human BNP 5′ flanking sequence conferred an ET-1 inducibility on the reporter gene. However, it did not confer CT-1 inducibility. These results show that distinct mechanisms are involved in BNP gene induction by ET-1 or CT-1, and (in this study) that the CT-1 responsive element is not located in the region examined.
AB - Cardiotrophin-1 (CT-1) a novel IL-6-related cytokine, induces distinct hypertrophic responses to endothelin-1 (ET-1) on cultured neonatal rat cardiac myocytes. We found that ET-1 and CT-1 show a distinct pattern of gene induction of natriuretic peptides. Elucidation of the transcriptional mechanisms of brain natriuretic peptide (BNP) gene induction by ET-1 or CT-1 will provide better information for our understanding of the molecular mechanisms of cardiac hypertrophy. In this study, reporter constructs containing the human BNP 5′ flanking sequence were transfected into neonatal rat cardiac myocytes and the cells were stimulated with ET-1 or CT-1. A total of 1813 bp of the human BNP 5′ flanking sequence conferred an ET-1 inducibility on the reporter gene. However, it did not confer CT-1 inducibility. These results show that distinct mechanisms are involved in BNP gene induction by ET-1 or CT-1, and (in this study) that the CT-1 responsive element is not located in the region examined.
UR - http://www.scopus.com/inward/record.url?scp=0000434445&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0000434445&partnerID=8YFLogxK
U2 - 10.1097/00005344-199800001-00099
DO - 10.1097/00005344-199800001-00099
M3 - Article
C2 - 9595480
AN - SCOPUS:0000434445
VL - 31
JO - Journal of Cardiovascular Pharmacology
JF - Journal of Cardiovascular Pharmacology
SN - 0160-2446
IS - SUPPL. 1
ER -