Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms

Koichiro Kuwahara, Yoshihiko Saito, Yoshihiro Ogawa, Naohisa Tamura, Masahiro Ishikawa, Masaki Harada, Emiko Ogawa, Yoshihiro Miyamoto, Ichiro Hamanaka, Shigeki Kamitani, Noboru Kajiyama, Nobuki Takahashi, Osamu Nakagawa, Izuru Masuda, Kazuwa Nakao

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33 Citations (Scopus)

Abstract

Cardiotrophin-1 (CT-1) a novel IL-6-related cytokine, induces distinct hypertrophic responses to endothelin-1 (ET-1) on cultured neonatal rat cardiac myocytes. We found that ET-1 and CT-1 show a distinct pattern of gene induction of natriuretic peptides. Elucidation of the transcriptional mechanisms of brain natriuretic peptide (BNP) gene induction by ET-1 or CT-1 will provide better information for our understanding of the molecular mechanisms of cardiac hypertrophy. In this study, reporter constructs containing the human BNP 5′ flanking sequence were transfected into neonatal rat cardiac myocytes and the cells were stimulated with ET-1 or CT-1. A total of 1813 bp of the human BNP 5′ flanking sequence conferred an ET-1 inducibility on the reporter gene. However, it did not confer CT-1 inducibility. These results show that distinct mechanisms are involved in BNP gene induction by ET-1 or CT-1, and (in this study) that the CT-1 responsive element is not located in the region examined.

Original languageEnglish
JournalJournal of Cardiovascular Pharmacology
Volume31
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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    Kuwahara, K., Saito, Y., Ogawa, Y., Tamura, N., Ishikawa, M., Harada, M., Ogawa, E., Miyamoto, Y., Hamanaka, I., Kamitani, S., Kajiyama, N., Takahashi, N., Nakagawa, O., Masuda, I., & Nakao, K. (1998). Endothelin-1 and cardiotrophin-1 induce brain natriuretic peptide gene expression by distinct transcriptional mechanisms. Journal of Cardiovascular Pharmacology, 31(SUPPL. 1). https://doi.org/10.1097/00005344-199800001-00099