TY - JOUR
T1 - Endothelin-1 is not involved in serotonin-induced coronary spasm in a swine model
AU - Fukai, Tohru
AU - Egashira, Kensuke
AU - Numaguchi, Kohtaro
AU - Hata, Hiroshi
AU - Takahashi, Teisuke
AU - Kasuya, Hiromitsu
AU - Sakata, Makoto
AU - Shimokawa, Hiroaki
AU - Takeshita, Akira
N1 - Funding Information:
This study was supported by a Grand-in-Aid for General Scientific Research from the Ministry of Education, Science and Culture, Tokyo, a Research Grant from the Naito Memorial Foundation, Tokyo, a Research Grant from the Uehara Memorial Foundation, Tokyo, and a Research Grant from the Japan Cardiovascular Foundation, Osaka.
PY - 1995
Y1 - 1995
N2 - Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4%) but not at the non-denuded control site (19 ± 4%, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3%, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.
AB - Objectives: The role of endothelin-1 (ET-1) in the pathogenesis of coronary artery spasm is not well understood. We aimed to determine if ET-1 is involved in serotonin-induced coronary spasm in the swine model. Methods: In 10 miniature pigs, a segment of the left anterior descending coronary artery was denuded and irradiated with X-ray. Three months after endothelial denudation, coronary vasomotion was assessed in vivo by quantitative arteriography. Results: Intracoronary serotonin at 10 μg/kg provoked coronary spasm (augmented narrowing of the luminal diameter) at the denuded site (diameter reduction 93 ± 4%) but not at the non-denuded control site (19 ± 4%, P < 0.01) associated with ST segment elevation in the region perfused by the denuded artery. Intracoronary administration of ET-1 at 25 ng/kg caused mild vasoconstriction of the denuded (26 ± 4) and non-denuded site (16 ± 3%, n.s.), but provoked ST segment elevation in the regions perfused by both the denuded and non-denuded arteries. The treatment with an endothelin antagonist (BQ123 0.1 mg/kg) significantly attenuated coronary vasoconstriction and ST segment elevation evoked with ET-1, but did not alter serotonin-induced vasoconstriction either at the denuded or control site. Conclusions: The results of this study suggest that endogenous ET-1 may not be involved in the pathogenesis of serotonin-induced coronary spasm in our swine model.
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U2 - 10.1016/S0008-6363(95)00022-4
DO - 10.1016/S0008-6363(95)00022-4
M3 - Article
C2 - 7585805
AN - SCOPUS:0029118961
SN - 0008-6363
VL - 30
SP - 193
EP - 199
JO - Cardiovascular Research
JF - Cardiovascular Research
IS - 2
ER -