Endothelin-3 activates a voltage-gated Ca channel via a pertussis toxin sensitive mechanism leading to dopamine release from PC12 cells

Schuichi Koizumi, Kazuhide Inoue, Yasufumi Kataoka, Masami Niwa, Akira Takanaka

Research output: Contribution to journalArticle

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Abstract

We have investigated the role of endothelin-3 (ET-3) in the stimulus-secretion coupling mechanism in rat pheochromocytoma PC12 cells. ET-3 (10-100 nM) evoked both dopamine (DA) release and an increase in intracellular Ca2+ concentration ([Ca]i). The ET-3-evoked DA release was partially inhibited by pretreatment with pertussis toxin (PTX; 2 ng/nl, 20 h). The release was also attenuated by the voltage-gated Ca channel (VGC) blockers Cd2+ (300 μM) or nicardipine (30 μM) and was completely abolished when external Ca2+ was removed. ET-3-evoked [Ca]i increase was attenuated by the application of these VGC blockers and by pretreatment with PTX, and was abolished by removal of extracellular Ca2+. Removal of external Na+ had no effect on either response. In light of these findings, we conclude that ET-3 evokes both DA release and an increase in [Ca]i by a mechanism which involves the activation of PTX-sensitive VGCs and the resultant influx of Ca2+.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalNeuroscience Letters
Volume166
Issue number2
DOIs
Publication statusPublished - Jan 31 1994
Externally publishedYes

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Endothelin-3
PC12 Cells
Pertussis Toxin
Dopamine
Nicardipine
Pheochromocytoma

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Endothelin-3 activates a voltage-gated Ca channel via a pertussis toxin sensitive mechanism leading to dopamine release from PC12 cells. / Koizumi, Schuichi; Inoue, Kazuhide; Kataoka, Yasufumi; Niwa, Masami; Takanaka, Akira.

In: Neuroscience Letters, Vol. 166, No. 2, 31.01.1994, p. 191-194.

Research output: Contribution to journalArticle

Koizumi, Schuichi ; Inoue, Kazuhide ; Kataoka, Yasufumi ; Niwa, Masami ; Takanaka, Akira. / Endothelin-3 activates a voltage-gated Ca channel via a pertussis toxin sensitive mechanism leading to dopamine release from PC12 cells. In: Neuroscience Letters. 1994 ; Vol. 166, No. 2. pp. 191-194.
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