Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta

Yusuke Ushida, Toshiro Matsui, Mitsuru Tanaka, Kiyoshi Matsumoto, Hirokazu Hosoyama, Atsuhiro Mitomi, Yuko Sagesaka, Takami Kakuda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The aim of this study was to point out the potential of tartary buckwheat on vascular functions. A nonabsorbed fraction of hot-water extract of tartary buckwheat on a SP70 column (TBSP-T), which was free from rutin, was used for this aim. In a contractile experiment using Sprague-Dawley rat thoracic aorta rings contracted by 1.0 μM phenylephrine (PE) or 50 mM KCl, TBSP-T evoked a significant vasorelaxation [EC50 (mg/ml): PE; 2.2; KCl, 1.9]. By a further fractionation of TBSP-T by liquid-liquid partitioning into basic, neutral and acidic fractions, a marked enhancement of vasorelaxation effect was observed only for acidic fraction (EC50, 0.25 mg/ml). The action of acidic fraction was significantly attenuated in endothelium-denuded aortic rings and in the presence of nitric oxide synthase inhibitor, NG-monomethyl-l-arginine (100 μM). The fraction also enhanced the cyclic guanosine monophosphate (cGMP) production in aortic rings contracted with PE [cGMP (pmol/mg protein): PE, 7.2±2.3; PE+Acidic fraction, 35±8]. These results indicate that acidic fraction could mediate NO/cGMP pathways, thereby exerting endothelium-dependent vasorelaxation action. In conclusion, tartary buckwheat was proven to regulate vascular tones and have latent acidic candidates except for rutin.

Original languageEnglish
Pages (from-to)700-707
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume19
Issue number10
DOIs
Publication statusPublished - Oct 1 2008

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Fagopyrum
Rutin
Phenylephrine
Thoracic Aorta
Vasodilation
Endothelium
Rats
Cyclic GMP
Blood Vessels
Liquids
Fractionation
Nitric Oxide Synthase
Sprague Dawley Rats
Arginine
Water
Proteins
Experiments

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta. / Ushida, Yusuke; Matsui, Toshiro; Tanaka, Mitsuru; Matsumoto, Kiyoshi; Hosoyama, Hirokazu; Mitomi, Atsuhiro; Sagesaka, Yuko; Kakuda, Takami.

In: Journal of Nutritional Biochemistry, Vol. 19, No. 10, 01.10.2008, p. 700-707.

Research output: Contribution to journalArticle

Ushida, Yusuke ; Matsui, Toshiro ; Tanaka, Mitsuru ; Matsumoto, Kiyoshi ; Hosoyama, Hirokazu ; Mitomi, Atsuhiro ; Sagesaka, Yuko ; Kakuda, Takami. / Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta. In: Journal of Nutritional Biochemistry. 2008 ; Vol. 19, No. 10. pp. 700-707.
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