Energy metabolism during cold ischemia and reperfusion in rat small intestinal transplantation: Comparison of jejunal and ileal grafts

Makoto Nakao, Tomoaki Taguchi, Keiko Yanai, Takaharu Yamada, Sachiyo Suita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background/Purpose: In segmental small intestinal transplantation, the question as to whether the jejunum or ileum is the better graft remains unclear. The authors investigated this question regarding nutrition, blood chemistry, and adaptation. Methods: The authors compared the jejunum and ileum as the proper portion of segmental graft in rat small intestinal transplantation, regarding energy metabolism both during cold preservation and after reperfusion using high-performance liquid chromatography, as well as histological examination. Results: In a cold preservation study, the concentrations of adenosine triphosphate (ATP) in the jejunum versus ileum at 0, 6, 12, 24, and 48 hours of cold preservation were 1.98 ± 0.93 versus 1.83 ± 0.84, 0.79 ± 0.51 versus 0.55 ± 0.41, 0.60 ± 0.41 versus 0.58 ± 0.45, 0.38 ± 0.28 versus 0.47 ± 0.39, and 0.44 ± 0.26 versus 0.55 ± 0.29 (μmol/g weight), respectively. There were no significant differences between the jejunum and ileum at any times of preservation. Adenosine monophosphate (AMP), adenosine diphosphate (ADP), total adenine nucleotides (TAN), and energy charge (EC) also showed no significant differences between the jejunum and ileum at any times of preservation. In the reperfusion study, the concentrations of ATP in jejunum versus ileum 30 minutes after reperfusion after 6, 12, and 24 hours of cold preservation were 0.68 ± 0.19 versus 0.50 ± 0.17, 0.71 ± 0.51 versus 0.42 ± 0.14, and 0.93 ± 0.29 versus 0.75 ± 0.47 (μmol/g dry weight), respectively. Each value was slightly higher in the jejunum than that in the ileum; however, there were no statistically significant differences. TAN showed the same changes as ATP. No significant differences were found between the jejunum and ileum in either AMP, ADP, or EC. The histological findings both just before and 30 minutes after reperfusion were also compared. However, no evident differences were found between the jejunum and ileum. Conclusion: These results thus suggest that no significant differences exist between the jejunum and ileum regarding energy metabolism either during cold preservation or after reperfusion in small intestinal transplantation.

Original languageEnglish
Pages (from-to)1675-1678
Number of pages4
JournalJournal of Pediatric Surgery
Volume32
Issue number12
DOIs
Publication statusPublished - Jan 1 1997

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Cold Ischemia
Jejunum
Ileum
Energy Metabolism
Reperfusion
Transplantation
Transplants
Adenine Nucleotides
Adenosine Triphosphate
Weights and Measures
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

Cite this

Energy metabolism during cold ischemia and reperfusion in rat small intestinal transplantation : Comparison of jejunal and ileal grafts. / Nakao, Makoto; Taguchi, Tomoaki; Yanai, Keiko; Yamada, Takaharu; Suita, Sachiyo.

In: Journal of Pediatric Surgery, Vol. 32, No. 12, 01.01.1997, p. 1675-1678.

Research output: Contribution to journalArticle

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abstract = "Background/Purpose: In segmental small intestinal transplantation, the question as to whether the jejunum or ileum is the better graft remains unclear. The authors investigated this question regarding nutrition, blood chemistry, and adaptation. Methods: The authors compared the jejunum and ileum as the proper portion of segmental graft in rat small intestinal transplantation, regarding energy metabolism both during cold preservation and after reperfusion using high-performance liquid chromatography, as well as histological examination. Results: In a cold preservation study, the concentrations of adenosine triphosphate (ATP) in the jejunum versus ileum at 0, 6, 12, 24, and 48 hours of cold preservation were 1.98 ± 0.93 versus 1.83 ± 0.84, 0.79 ± 0.51 versus 0.55 ± 0.41, 0.60 ± 0.41 versus 0.58 ± 0.45, 0.38 ± 0.28 versus 0.47 ± 0.39, and 0.44 ± 0.26 versus 0.55 ± 0.29 (μmol/g weight), respectively. There were no significant differences between the jejunum and ileum at any times of preservation. Adenosine monophosphate (AMP), adenosine diphosphate (ADP), total adenine nucleotides (TAN), and energy charge (EC) also showed no significant differences between the jejunum and ileum at any times of preservation. In the reperfusion study, the concentrations of ATP in jejunum versus ileum 30 minutes after reperfusion after 6, 12, and 24 hours of cold preservation were 0.68 ± 0.19 versus 0.50 ± 0.17, 0.71 ± 0.51 versus 0.42 ± 0.14, and 0.93 ± 0.29 versus 0.75 ± 0.47 (μmol/g dry weight), respectively. Each value was slightly higher in the jejunum than that in the ileum; however, there were no statistically significant differences. TAN showed the same changes as ATP. No significant differences were found between the jejunum and ileum in either AMP, ADP, or EC. The histological findings both just before and 30 minutes after reperfusion were also compared. However, no evident differences were found between the jejunum and ileum. Conclusion: These results thus suggest that no significant differences exist between the jejunum and ileum regarding energy metabolism either during cold preservation or after reperfusion in small intestinal transplantation.",
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AU - Nakao, Makoto

AU - Taguchi, Tomoaki

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AU - Yamada, Takaharu

AU - Suita, Sachiyo

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