Engineered Coatings for Titanium Implants to Present Ultralow Doses of BMP-7

Mohammed Al-Jarsha, Vladimíra Moulisová, Aldo Leal-Egaña, Andrew Connell, Kurt B. Naudi, Ashraf F. Ayoub, Matthew J. Dalby, Manuel Salmerón-Sánchez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The ongoing research to improve the clinical outcome of titanium implants has resulted in the implemetation of multiple approches to deliver osteogenic growth factors accelerating and sustaining osseointegration. Here we show the presentation of human bone morphogenetic protein 7 (BMP-7) adsorbed to titanium discs coated with poly(ethyl acrylate) (PEA). We have previously shown that PEA promotes fibronectin organization into nanonetworks exposing integrin- and growth-factor-binding domains, allowing a synergistic interaction at the integrin/growth factor receptor level. Here, titanium discs were coated with PEA and fibronectin and then decorated with ng/mL doses of BMP-7. Human mesenchymal stem cells were used to investigate cellular responses on these functionalized microenvironments. Cell adhesion, proliferation, and mineralization, as well as osteogenic markers expression (osteopontin and osteocalcin) revealed the ability of the system to be more potent in osteodifferentiation of the mesenchymal cells than combinations of titanium and BMP-7 in absence of PEA coatings. This work represents a novel strategy to improve the biological activity of titanium implants with BMP-7.

Original languageEnglish
Pages (from-to)1812-1819
Number of pages8
JournalACS Biomaterials Science and Engineering
Volume4
Issue number5
DOIs
Publication statusPublished - May 14 2018

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Al-Jarsha, M., Moulisová, V., Leal-Egaña, A., Connell, A., Naudi, K. B., Ayoub, A. F., ... Salmerón-Sánchez, M. (2018). Engineered Coatings for Titanium Implants to Present Ultralow Doses of BMP-7. ACS Biomaterials Science and Engineering, 4(5), 1812-1819. https://doi.org/10.1021/acsbiomaterials.7b01037