Engineered Nanogel Particles Enhance the Photoautotrophic Biosynthesis of Polyhydroxyalkanoate in Marine Photosynthetic Bacteria

Pisanee Srisawat, Mieko Higuchi-Takeuchi, Ryutaro Honda, Tomokazu Shirai, Akihiko Kondo, Yu Hoshino, Keiji Numata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Improving polyhydroxyalkanoate (PHA, a biodegradable plastic) production under photoautotrophic cultivation is challenging for sustainable bioproduction. In this study, we demonstrated the use of engineered nanogel particles to enhance PHA accumulation in the marine photosynthetic bacterium Rhodovulum sulfidophilum under photoautotrophic culture. We screened the effect of 13 engineered nanogel particles on the cell growth and PHA accumulation of R. sulfidophilum. The addition of anionic nanogel particles significantly enhanced PHA accumulation in R. sulfidophilum up to 157-fold compared to that without nanogel particles. By performing 13C tracer experiments and gas chromatography-mass spectrometry analysis, we confirmed that HCO3- was assimilated throughout the central carbon metabolism and that the accumulated PHA was indeed incorporated from HCO3-. Our results indicate successful PHA production with the supplementation of engineered nanogel particles under photoautotrophic cultivation in R. sulfidophilum. Furthermore, the strategy of using engineered nanoparticles demonstrated in this study may be applicable to other microbial cell factories to produce other commodity metabolites.

Original languageEnglish
Pages (from-to)4133-4142
Number of pages10
JournalACS Sustainable Chemistry and Engineering
Volume10
Issue number13
DOIs
Publication statusPublished - Apr 4 2022

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment

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