@article{283e5fd3d5c14dd8977705ac59f17a88,
title = "Engineered protein-tag for rapid live-cell fluorogenic visualization of proteins by anionic probes",
abstract = "Live-cell protein labeling using a protein tag and a fluorescent probe is a powerful approach for studying protein localization and dynamics. Herein, we engineered a protein tag, PYP-tag, to image membrane proteins with high contrast. Mutations of charged amino acids on the surface of PYP-tag led to the enhancement of labeling kinetics and brightness. Furthermore, the modification of the surface charge did not affect its expression on the cell surface, and we succeeded in applying this labeling technique to high-contrast imaging of translocation of glucose transporter 4.",
author = "Jingchi Gao and Yuichiro Hori and Miyako Nishiura and Mathieu Bordy and Jens Hasserodt and Kazuya Kikuchi",
note = "Funding Information: This research was supported by JSPS KAKENHI (Grant Numbers: JP17H06409 “Frontier Research on Chemical Communications”, JP18H03935, JP25220207, JP19K22255 to K. K.; JP17H02210, JP17H06005, JP18K19402, JP18H04735 “Resonance Bio” to Y. H.; and JP19J12178 to J. G.), by JSPS Asian CORE Program, “Asian Chemical Biology Initiative”, by JSPS A3 Foresight Program, by the Japan Agency for Medical Research and Development (AMED, No. 18he0902005h0004, No. 17ae0101041h9902, No. 18fm0208018h0002 to K. K.), by JST SICORP (Grant Number: JPMJSC1602), by the Takeda Science Foundation, by the Asahi Glass Foundation, and by the French National Research Agency (ANR-16-JTIC-0001-01 to J. H.). Publisher Copyright: {\textcopyright} 2020 The Chemical Society of Japan",
year = "2020",
doi = "10.1246/cl.190875",
language = "English",
volume = "49",
pages = "232--235",
journal = "Chemistry Letters",
issn = "0366-7022",
publisher = "The Chemical Society of Japan",
number = "3",
}