Engineered synthetic polymer nanoparticles as IgG affinity ligands

Shih Hui Lee, Yu Hoshino, Arlo Randall, Zhiyang Zeng, Piere Baldi, Ruey An Doong, Kenneth J. Shea

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

A process for the preparation of an abiotic protein affinity ligand is described. The affinity ligand, a synthetic polymer hydrogel nanoparticle (NP), is formulated with functional groups complementary to the surface presentation of the target protein. An iterative process is used to improve affinity by optimizing the composition and proportion of functional monomers. Since the polymer NPs are formed by a kinetically driven process, the sequence of functional monomers in the polymer chain is not controlled; only the average composition can be adjusted by the stoichiometry of the monomers in the feed. To compensate for this the hydrogel NP is lightly cross-linked resulting in chain flexibility that takes place on a submillisecond time scale allowing the polymer to "map" onto a protein surface with complementary functionality. In this study, we report a lightly cross-linked (2%) N-isopropyl acrylamide (NIPAm) synthetic polymer NP (50-65 nm) incorporating hydrophobic and carboxylate groups that binds with high affinity to the Fc fragment of IgG. The affinity and amount of NP bound to IgG is pH dependent. The hydrogel NP inhibits protein A binding to the Fc domain at pH 5.5, but not at pH 7.3. A computational analysis was used to identify potential NP-protein interaction sites. Candidates include a NP binding domain that overlaps with the protein A-Fc binding domain at pH 5.5. The computational analysis supports the inhibition experimental results and is attributed to the difference in the charged state of histidine residues. Affinity of the NP (3.5-8.5 nM) to the Fc domain at pH 5.5 is comparable to protein A at pH 7. These results establish that engineered synthetic polymer NPs can be formulated with an intrinsic affinity to a specific domain of a large biomacromolecule.

Original languageEnglish
Pages (from-to)15765-15772
Number of pages8
JournalJournal of the American Chemical Society
Volume134
Issue number38
DOIs
Publication statusPublished - Sep 26 2012

Fingerprint

Nanoparticles
Polymers
Immunoglobulin G
Ligands
Proteins
Hydrogel
Staphylococcal Protein A
Hydrogels
Monomers
Immunoglobulin Fc Fragments
Acrylamide
Chemical analysis
Histidine
Protein Binding
Stoichiometry
Functional groups
Membrane Proteins

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Lee, S. H., Hoshino, Y., Randall, A., Zeng, Z., Baldi, P., Doong, R. A., & Shea, K. J. (2012). Engineered synthetic polymer nanoparticles as IgG affinity ligands. Journal of the American Chemical Society, 134(38), 15765-15772. https://doi.org/10.1021/ja303612d

Engineered synthetic polymer nanoparticles as IgG affinity ligands. / Lee, Shih Hui; Hoshino, Yu; Randall, Arlo; Zeng, Zhiyang; Baldi, Piere; Doong, Ruey An; Shea, Kenneth J.

In: Journal of the American Chemical Society, Vol. 134, No. 38, 26.09.2012, p. 15765-15772.

Research output: Contribution to journalArticle

Lee, SH, Hoshino, Y, Randall, A, Zeng, Z, Baldi, P, Doong, RA & Shea, KJ 2012, 'Engineered synthetic polymer nanoparticles as IgG affinity ligands', Journal of the American Chemical Society, vol. 134, no. 38, pp. 15765-15772. https://doi.org/10.1021/ja303612d
Lee, Shih Hui ; Hoshino, Yu ; Randall, Arlo ; Zeng, Zhiyang ; Baldi, Piere ; Doong, Ruey An ; Shea, Kenneth J. / Engineered synthetic polymer nanoparticles as IgG affinity ligands. In: Journal of the American Chemical Society. 2012 ; Vol. 134, No. 38. pp. 15765-15772.
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