Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light

Tamami Kusakabe, Tsuneyuki Tatsuke, Keigo Tsuruno, Yasutaka Hirokawa, Shota Atsumi, James C. Liao, Taizo Hanai

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Production of alternate fuels or chemicals directly from solar energy and carbon dioxide using engineered cyanobacteria is an attractive method to reduce petroleum dependency and minimize carbon emissions. Here, we constructed a synthetic pathway composed of acetyl-CoA acetyl transferase (encoded by thl), acetoacetyl-CoA transferase (encoded by atoAD), acetoacetate decarboxylase (encoded by adc) and secondary alcohol dehydrogenase (encoded by adh) in Synechococcus elongatus strain PCC 7942 to produce isopropanol. The enzyme-coding genes, heterogeneously originating from Clostridium acetobutylicum ATCC 824 (thl and adc), Escherichia coli K-12 MG1655 (atoAD) and Clostridium beijerinckii (adh), were integrated into the S. elongatus genome. Under the optimized production conditions, the engineered cyanobacteria produced 26.5. mg/L of isopropanol after 9 days.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalMetabolic Engineering
Volume20
DOIs
Publication statusPublished - Nov 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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