Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device

Yuji Nashimoto, Itsuki Kunita, Akiko Nakamasu, Yu Suke Torisawa, Masamune Nakayama, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we developed a microfluidic platform for a three-dimensional tissue model with a perfusable capillary network, which will allow, for the first time, a perfusion-culture in a tissue model with a high cell density. Our group previously reported that a spheroid of lung fibroblasts induced angiogenic sprouts from microchannels [1]. In this study, we successfully connected angiogenic sprouts to the vessel-like hollow structure in a spheroid and perfused the formed vascular network through microfluidic channels to the spheroid. This model opens up new techniques for tissue-culture for long-term.

Original languageEnglish
Title of host publication2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages592-595
Number of pages4
ISBN (Electronic)9781509050789
DOIs
Publication statusPublished - Feb 23 2017
Event30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
Duration: Jan 22 2017Jan 26 2017

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
CountryUnited States
CityLas Vegas
Period1/22/171/26/17

Fingerprint

microfluidic devices
spheroids
Microfluidics
engineering
Tissue
Tissue culture
fibroblasts
Fibroblasts
microchannels
Microchannels
lungs
vessels
hollow
platforms
cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Nashimoto, Y., Kunita, I., Nakamasu, A., Torisawa, Y. S., Nakayama, M., Kotera, H., ... Yokokawa, R. (2017). Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 592-595). [7863476] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863476

Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. / Nashimoto, Yuji; Kunita, Itsuki; Nakamasu, Akiko; Torisawa, Yu Suke; Nakayama, Masamune; Kotera, Hidetoshi; Nishiyama, Koichi; Miura, Takashi; Yokokawa, Ryuji.

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 592-595 7863476.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nashimoto, Y, Kunita, I, Nakamasu, A, Torisawa, YS, Nakayama, M, Kotera, H, Nishiyama, K, Miura, T & Yokokawa, R 2017, Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. in 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863476, Institute of Electrical and Electronics Engineers Inc., pp. 592-595, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017, Las Vegas, United States, 1/22/17. https://doi.org/10.1109/MEMSYS.2017.7863476
Nashimoto Y, Kunita I, Nakamasu A, Torisawa YS, Nakayama M, Kotera H et al. Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 592-595. 7863476 https://doi.org/10.1109/MEMSYS.2017.7863476
Nashimoto, Yuji ; Kunita, Itsuki ; Nakamasu, Akiko ; Torisawa, Yu Suke ; Nakayama, Masamune ; Kotera, Hidetoshi ; Nishiyama, Koichi ; Miura, Takashi ; Yokokawa, Ryuji. / Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 592-595
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