Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device

Yuji Nashimoto, Itsuki Kunita, Akiko Nakamasu, Yu Suke Torisawa, Masamune Nakayama, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we developed a microfluidic platform for a three-dimensional tissue model with a perfusable capillary network, which will allow, for the first time, a perfusion-culture in a tissue model with a high cell density. Our group previously reported that a spheroid of lung fibroblasts induced angiogenic sprouts from microchannels [1]. In this study, we successfully connected angiogenic sprouts to the vessel-like hollow structure in a spheroid and perfused the formed vascular network through microfluidic channels to the spheroid. This model opens up new techniques for tissue-culture for long-term.

Original languageEnglish
Title of host publication2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages592-595
Number of pages4
ISBN (Electronic)9781509050789
DOIs
Publication statusPublished - Feb 23 2017
Event30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017 - Las Vegas, United States
Duration: Jan 22 2017Jan 26 2017

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
CountryUnited States
CityLas Vegas
Period1/22/171/26/17

Fingerprint

microfluidic devices
spheroids
Microfluidics
engineering
Tissue
Tissue culture
fibroblasts
Fibroblasts
microchannels
Microchannels
lungs
vessels
hollow
platforms
cells

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Nashimoto, Y., Kunita, I., Nakamasu, A., Torisawa, Y. S., Nakayama, M., Kotera, H., ... Yokokawa, R. (2017). Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 (pp. 592-595). [7863476] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2017.7863476

Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. / Nashimoto, Yuji; Kunita, Itsuki; Nakamasu, Akiko; Torisawa, Yu Suke; Nakayama, Masamune; Kotera, Hidetoshi; Nishiyama, Koichi; Miura, Takashi; Yokokawa, Ryuji.

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 592-595 7863476 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nashimoto, Y, Kunita, I, Nakamasu, A, Torisawa, YS, Nakayama, M, Kotera, H, Nishiyama, K, Miura, T & Yokokawa, R 2017, Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. in 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017., 7863476, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 592-595, 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017, Las Vegas, United States, 1/22/17. https://doi.org/10.1109/MEMSYS.2017.7863476
Nashimoto Y, Kunita I, Nakamasu A, Torisawa YS, Nakayama M, Kotera H et al. Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. In 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 592-595. 7863476. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2017.7863476
Nashimoto, Yuji ; Kunita, Itsuki ; Nakamasu, Akiko ; Torisawa, Yu Suke ; Nakayama, Masamune ; Kotera, Hidetoshi ; Nishiyama, Koichi ; Miura, Takashi ; Yokokawa, Ryuji. / Engineering a three-dimensional tissue model with a perfusable vasculature in a microfluidic device. 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 592-595 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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