Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery

Hiroyuki Koide, Tatsuya Fukuta, Anna Okishim, Saki Ariizumi, Chiaki Kiyokawa, Hiroki Tsuchida, Masahiko Nakamoto, Keiichi Yoshimatsu, Hidenori Ando, Takehisa Dewa, Tomohiro Asai, Naoto Oku, Yu Hoshino, Kenneth J. Shea

Research output: Contribution to journalArticle

Abstract

The affinity of a synthetic polymer nanoparticle (NP) to a target biomacromolecule is determined by the association and dissociation rate constants (kon, koff) of the interaction. The individual rates and their sensitivity to local environmental influences are important factors for the on-demand capture and release a target biomacromolecule. Positively charged NPs for small interfering RNA (siRNA) delivery is a case in point. The knockdown efficacy of siRNA can be strongly influenced by the binding kinetics to the NP. Here, we show that kon and koff of siRNA to NPs can be individually engineered by tuning the chemical structure and composition of the NP. N-Isopropylacrylamide-based NPs functionalized with hydrophobic and amine monomers were used. koff decreased by increasing the amount of amine groups in the NP, whereas kon did not change. Importantly, NPs showing a low koff at pH 5.5 together with a high koff at pH 7.4 showed high knockdown efficiency when NP/siRNA complexes were packaged in lipid nanoparticles. These results provide direct evidence for the premise that the efficacy of an siRNA delivery vector is linked with the strong affinity to the siRNA in the endosome and low affinity in the cytoplasm.

Original languageEnglish
Pages (from-to)3648-3657
Number of pages10
JournalBiomacromolecules
Volume20
Issue number10
DOIs
Publication statusPublished - Oct 14 2019

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RNA
Small Interfering RNA
Polymers
Nanoparticles
Kinetics
Amines
Lipids
Rate constants
Tuning
Monomers
Association reactions
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Koide, H., Fukuta, T., Okishim, A., Ariizumi, S., Kiyokawa, C., Tsuchida, H., ... Shea, K. J. (2019). Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery. Biomacromolecules, 20(10), 3648-3657. https://doi.org/10.1021/acs.biomac.9b00611

Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery. / Koide, Hiroyuki; Fukuta, Tatsuya; Okishim, Anna; Ariizumi, Saki; Kiyokawa, Chiaki; Tsuchida, Hiroki; Nakamoto, Masahiko; Yoshimatsu, Keiichi; Ando, Hidenori; Dewa, Takehisa; Asai, Tomohiro; Oku, Naoto; Hoshino, Yu; Shea, Kenneth J.

In: Biomacromolecules, Vol. 20, No. 10, 14.10.2019, p. 3648-3657.

Research output: Contribution to journalArticle

Koide, H, Fukuta, T, Okishim, A, Ariizumi, S, Kiyokawa, C, Tsuchida, H, Nakamoto, M, Yoshimatsu, K, Ando, H, Dewa, T, Asai, T, Oku, N, Hoshino, Y & Shea, KJ 2019, 'Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery', Biomacromolecules, vol. 20, no. 10, pp. 3648-3657. https://doi.org/10.1021/acs.biomac.9b00611
Koide H, Fukuta T, Okishim A, Ariizumi S, Kiyokawa C, Tsuchida H et al. Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery. Biomacromolecules. 2019 Oct 14;20(10):3648-3657. https://doi.org/10.1021/acs.biomac.9b00611
Koide, Hiroyuki ; Fukuta, Tatsuya ; Okishim, Anna ; Ariizumi, Saki ; Kiyokawa, Chiaki ; Tsuchida, Hiroki ; Nakamoto, Masahiko ; Yoshimatsu, Keiichi ; Ando, Hidenori ; Dewa, Takehisa ; Asai, Tomohiro ; Oku, Naoto ; Hoshino, Yu ; Shea, Kenneth J. / Engineering the Binding Kinetics of Synthetic Polymer Nanoparticles for siRNA Delivery. In: Biomacromolecules. 2019 ; Vol. 20, No. 10. pp. 3648-3657.
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