Enhanced angiogenesis by transplantation of mesenchymal stem cell sheet created by a novel magnetic tissue engineering method

Masakazu Ishii, Rei Shibata, Yasushi Numaguchi, Tetsutaro Kito, Hirohiko Suzuki, Kazunori Shimizu, Akira Ito, Hiroyuki Honda, Toyoaki Murohara

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

OBJECTIVE-: Therapeutic angiogenesis with cell transplantation represents a novel strategy for severe ischemic diseases. However, some patients have poor response to such conventional injection-based angiogenic cell therapy. Here, we investigated a therapeutic potential of mesenchymal stem cell (MSC) sheet created by a novel magnetite tissue engineering technology for reparative angiogenesis. METHODS AND RESULTS-: Human MSCs incubated with magnetic nanoparticle-containing liposomes were cultured, and a magnet was placed on the reverse side. Magnetized MSCs formed multilayered cell sheets according to magnetic force. Nude mice were subjected to unilateral hind limb ischemia and separated into 3 groups. For the control group, saline was injected into ischemic tissue. In the MSC-injected group, mice received magnetized MSCs by conventional needle injections without sheet formula as a control cell group. In the MSC-sheet group, MSC sheet was layered onto the ischemic tissues before skin closure. Blood flow recovery and the extent of angiogenesis were assessed by a laser Doppler blood flowmetry and histological capillary density, respectively. The MSC-sheet group had a greater angiogenesis in ischemic tissues compared to the control and MSC-injected groups. The angiogenic and tissue-preserving effects of MSC sheets were attributable to an increased expression of vascular endothelial growth factor and reduced apoptosis in ischemic tissues. In cultured MSCs, magnetic labeling itself inhibited apoptosis via a catalase-like antioxidative mechanism. CONCLUSION-: MSC sheet created by the novel magnetic nanoparticle-based tissue engineering technology would represent a new modality for therapeutic angiogenesis and tissue regeneration.

Original languageEnglish
Pages (from-to)2210-2215
Number of pages6
JournalArteriosclerosis, thrombosis, and vascular biology
Volume31
Issue number10
DOIs
Publication statusPublished - Oct 1 2011

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Mesenchymal Stem Cell Transplantation
Tissue Engineering
Mesenchymal Stromal Cells
Nanoparticles
Ferrosoferric Oxide
Apoptosis
Technology
Control Groups
Laser-Doppler Flowmetry
Injections
Magnets
Cell Transplantation
Cell- and Tissue-Based Therapy
Nude Mice
Liposomes
Catalase
Vascular Endothelial Growth Factor A
Needles
Regeneration
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Enhanced angiogenesis by transplantation of mesenchymal stem cell sheet created by a novel magnetic tissue engineering method. / Ishii, Masakazu; Shibata, Rei; Numaguchi, Yasushi; Kito, Tetsutaro; Suzuki, Hirohiko; Shimizu, Kazunori; Ito, Akira; Honda, Hiroyuki; Murohara, Toyoaki.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 31, No. 10, 01.10.2011, p. 2210-2215.

Research output: Contribution to journalArticle

Ishii, Masakazu ; Shibata, Rei ; Numaguchi, Yasushi ; Kito, Tetsutaro ; Suzuki, Hirohiko ; Shimizu, Kazunori ; Ito, Akira ; Honda, Hiroyuki ; Murohara, Toyoaki. / Enhanced angiogenesis by transplantation of mesenchymal stem cell sheet created by a novel magnetic tissue engineering method. In: Arteriosclerosis, thrombosis, and vascular biology. 2011 ; Vol. 31, No. 10. pp. 2210-2215.
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