Abstract
Background and Purpose-The level of platelet-derived growth factor (PDGF) in cerebrospinal fluid is elevated in subarachnoid hemorrhage (SAH). Therefore, the contractile effect of PDGF on the basilar artery was examined in SAH. Methods and Results-A rabbit double-hemorrhage SAH model was used. In the medial layers of the control basilar artery, PDGF had no effect on contraction up to 1 nmol/L, whereas 3 nmol/L PDGF induced slight contraction. In SAH, PDGF induced an enhanced contraction with an increase in [Ca2+];at 1 nmol/L and higher concentrations. The levels of [Ca2+]iand tension induced by 1 nmol/L PDGF in SAH were 17% and 20%, respectively, of those obtained with 118 mmol/L K+ depolarization. The PDGF-induced elevation of [Ca2+]i and contraction seen in SAH were abolished in the absence of extracellular Ca2+. In α-toxin-permeabilized strips of SAH animals, PDGF induced no further development of tension during contraction induced by 300 nmol/L Ca2+, suggesting no direct effect on myofilament Ca2+ sensitivity. Genistein at 10 /μmol/L completely inhibited the tension induced by 1 nmol/L PDGF. The level of myosin light-chain phosphorylation was significantly increased by 1 nmol/L PDGF. Conclusions-These results show that the contractile response to PDGF of the basilar artery was enhanced in SAH. The PDGF-induced contraction depended mostly on tyrosine phosphorylation and Ca 2+-dependent myosin light-chain phosphorylation. The enhancement of the responsiveness to PDGF may therefore contribute to the development of cerebral vasospasm after SAH.
| Original language | English |
|---|---|
| Pages (from-to) | 591-596 |
| Number of pages | 6 |
| Journal | Stroke |
| Volume | 40 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2009 |
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All Science Journal Classification (ASJC) codes
- Clinical Neurology
- Cardiology and Cardiovascular Medicine
- Advanced and Specialised Nursing
Cite this
Enhanced contractile response of the basilar artery to platelet-derived growth factor in subarachnoid hemorrhage. / Maeda, Yoshihisa; Hirano, Katsuya; Hirano, Mayumi; Kikkawa, Yuichiro; Kameda, Katsuharu; Sasaki, Tomio; Kanaide, Hideo.
In: Stroke, Vol. 40, No. 2, 01.02.2009, p. 591-596.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced contractile response of the basilar artery to platelet-derived growth factor in subarachnoid hemorrhage
AU - Maeda, Yoshihisa
AU - Hirano, Katsuya
AU - Hirano, Mayumi
AU - Kikkawa, Yuichiro
AU - Kameda, Katsuharu
AU - Sasaki, Tomio
AU - Kanaide, Hideo
PY - 2009/2/1
Y1 - 2009/2/1
N2 - Background and Purpose-The level of platelet-derived growth factor (PDGF) in cerebrospinal fluid is elevated in subarachnoid hemorrhage (SAH). Therefore, the contractile effect of PDGF on the basilar artery was examined in SAH. Methods and Results-A rabbit double-hemorrhage SAH model was used. In the medial layers of the control basilar artery, PDGF had no effect on contraction up to 1 nmol/L, whereas 3 nmol/L PDGF induced slight contraction. In SAH, PDGF induced an enhanced contraction with an increase in [Ca2+];at 1 nmol/L and higher concentrations. The levels of [Ca2+]iand tension induced by 1 nmol/L PDGF in SAH were 17% and 20%, respectively, of those obtained with 118 mmol/L K+ depolarization. The PDGF-induced elevation of [Ca2+]i and contraction seen in SAH were abolished in the absence of extracellular Ca2+. In α-toxin-permeabilized strips of SAH animals, PDGF induced no further development of tension during contraction induced by 300 nmol/L Ca2+, suggesting no direct effect on myofilament Ca2+ sensitivity. Genistein at 10 /μmol/L completely inhibited the tension induced by 1 nmol/L PDGF. The level of myosin light-chain phosphorylation was significantly increased by 1 nmol/L PDGF. Conclusions-These results show that the contractile response to PDGF of the basilar artery was enhanced in SAH. The PDGF-induced contraction depended mostly on tyrosine phosphorylation and Ca 2+-dependent myosin light-chain phosphorylation. The enhancement of the responsiveness to PDGF may therefore contribute to the development of cerebral vasospasm after SAH.
AB - Background and Purpose-The level of platelet-derived growth factor (PDGF) in cerebrospinal fluid is elevated in subarachnoid hemorrhage (SAH). Therefore, the contractile effect of PDGF on the basilar artery was examined in SAH. Methods and Results-A rabbit double-hemorrhage SAH model was used. In the medial layers of the control basilar artery, PDGF had no effect on contraction up to 1 nmol/L, whereas 3 nmol/L PDGF induced slight contraction. In SAH, PDGF induced an enhanced contraction with an increase in [Ca2+];at 1 nmol/L and higher concentrations. The levels of [Ca2+]iand tension induced by 1 nmol/L PDGF in SAH were 17% and 20%, respectively, of those obtained with 118 mmol/L K+ depolarization. The PDGF-induced elevation of [Ca2+]i and contraction seen in SAH were abolished in the absence of extracellular Ca2+. In α-toxin-permeabilized strips of SAH animals, PDGF induced no further development of tension during contraction induced by 300 nmol/L Ca2+, suggesting no direct effect on myofilament Ca2+ sensitivity. Genistein at 10 /μmol/L completely inhibited the tension induced by 1 nmol/L PDGF. The level of myosin light-chain phosphorylation was significantly increased by 1 nmol/L PDGF. Conclusions-These results show that the contractile response to PDGF of the basilar artery was enhanced in SAH. The PDGF-induced contraction depended mostly on tyrosine phosphorylation and Ca 2+-dependent myosin light-chain phosphorylation. The enhancement of the responsiveness to PDGF may therefore contribute to the development of cerebral vasospasm after SAH.
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U2 - 10.1161/STROKEAHA.108.530196
DO - 10.1161/STROKEAHA.108.530196
M3 - Article
VL - 40
SP - 591
EP - 596
JO - Stroke
JF - Stroke
SN - 0039-2499
IS - 2
ER -