Enhanced electrophoretic resolution of monosulfate glycosaminoglycan disaccharide isomers on poly(methyl methacrylate) chips

Yong Zhang, Guichen Ping, Bingmei Zhu, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

To improve the separation of monosulfate glycosaminoglycan disaccharide isomers by microchip electrophoresis, we found that addition of 1,4-dioxane (DO) dramatically improved analyte resolution, probably due to solvation effects. Methylcellulose (MC) was tested for the ability to suppress EOF and analyte adsorption to the chip. To improve analyte resolution, buffer pH, β-CD, and DO were systematically investigated. Fast separation was achieved by increasing the electric field strength, and field-amplified sample stacking occurred with increasing buffer concentrations. Therefore, based on our findings, we describe an efficient method for the separation of mono-sulfate and trisulfate unsaturated disaccharides (ΔDi-UA2S, ΔDi-4S, ΔDi-6S, and ΔDi-triS) derivatized with 2-aminoacridone hydrochloride. A mixture of monosulfate disaccharide isomers (ΔDi-UA2S, ΔDi-4S, and ΔDi-6S) was baseline-separated within 75 s on a poly(methyl methacrylate) chip using a mixed buffer (DO /running buffer 57:43 v:v), 0.5% MC, pH 6.81, with an Esep of 558 V/cm. The theoretical plate was in the range of 5 × 105 to 1 × 106 m-1.

Original languageEnglish
Pages (from-to)414-421
Number of pages8
JournalELECTROPHORESIS
Volume28
Issue number3
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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