Enhanced lateral inhibition in the barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 in mice

Hiroki Toyoda, Mitsuru Saito, Hajime Sato, Tsutomu Kawano, Shinpei Kawakami, Hirofumi Yatani, Takashi Kanematsu, Masato Hirata, Youngnam Kang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We previously demonstrated that the deletion of phospholipase C-related catalytically inactive protein-1/2 (PRIP-1/2) enhances the desensitization of GABAA receptors (GABAARs), while it facilitates their resensitization at the offset of GABA puff, causing a hump-like tail current (tail-I) in layer 3 (L3) pyramidal cells (PCs) of the barrel cortex. In the present study, we investigated whether inhibitory synaptic transmission in L3 PCs in the barrel cortex is altered in the PRIP-1/2 double-knockout (PRIP-DKO) mice, and if so, how the interaction between excitation and inhibition is subsequently modified. PRIP-1/2 deletion resulted in the prolongation of the decay phase of inhibitory postsynaptic currents/potentials (IPSCs/IPSPs) in L3 PCs evoked by stimulation of L3, leaving the overall features of miniature IPSCs unchanged. An optical imaging revealed that the spatiotemporal profile of a horizontal excitation spread across columns in L2/3 caused by L4 stimulation in the barrel cortex was more restricted in PRIP-DKO mice compared to the wild type, while those obtained in the presence of bicuculline were almost identical between the two genotypes. These findings suggest that PRIP-1/2 deletion enhances the lateral inhibition by prolonging inhibitory synaptic actions to limit the intercolumnar integration in the barrel cortex. Considering the present findings together with our previous study including a mathematical simulation, the prolongation of inhibitory synaptic actions is likely to result from an enhancement of desensitization followed by an enhanced resensitization in GABAARs.

Original languageEnglish
Pages (from-to)1445-1456
Number of pages12
JournalPflugers Archiv European Journal of Physiology
Volume467
Issue number7
DOIs
Publication statusPublished - Jul 13 2015

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Type C Phospholipases
Inhibitory Postsynaptic Potentials
Pyramidal Cells
GABA-A Receptors
Knockout Mice
Tail
Proteins
Bicuculline
Optical Imaging
Synaptic Transmission
gamma-Aminobutyric Acid
Genotype
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Enhanced lateral inhibition in the barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 in mice. / Toyoda, Hiroki; Saito, Mitsuru; Sato, Hajime; Kawano, Tsutomu; Kawakami, Shinpei; Yatani, Hirofumi; Kanematsu, Takashi; Hirata, Masato; Kang, Youngnam.

In: Pflugers Archiv European Journal of Physiology, Vol. 467, No. 7, 13.07.2015, p. 1445-1456.

Research output: Contribution to journalArticle

Toyoda, Hiroki ; Saito, Mitsuru ; Sato, Hajime ; Kawano, Tsutomu ; Kawakami, Shinpei ; Yatani, Hirofumi ; Kanematsu, Takashi ; Hirata, Masato ; Kang, Youngnam. / Enhanced lateral inhibition in the barrel cortex by deletion of phospholipase C-related catalytically inactive protein-1/2 in mice. In: Pflugers Archiv European Journal of Physiology. 2015 ; Vol. 467, No. 7. pp. 1445-1456.
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