Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-β

Takeshi Nagasaki, Takeshi Kawazu, Taro Tachibana, Seizo Tamagaki, Seiji Shinkai

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In order to enhance the nuclear import of exogenous genes, novel plasmid DNA/importin-β conjugates, which consist of a biotinylated plasmid DNA and a recombinant streptavidin-fused importin-β, were prepared. The spacer length between plasmid DNA and biotin and the number of introduced biotin were adjusted. The microinjection of plasmid DNA/importin-β conjugates into the cytoplasm of NIH3T3 cells resulted in the nuclear localization of conjugates and the higher expression efficiency, compared to intact plasmid DNA alone. These results indicate that plasmid DNA/importin-β conjugates would be an important tool to enhance the nuclear localization of exogenous DNA in non-viral gene delivery system.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalJournal of Controlled Release
Volume103
Issue number1
DOIs
Publication statusPublished - Mar 2 2005

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Karyopherins
Streptavidin
Cell Nucleus Active Transport
Transfection
Plasmids
DNA
Biotin
Gene Transfer Techniques
Recombinant DNA
Microinjections
Cytoplasm
Genes

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-β. / Nagasaki, Takeshi; Kawazu, Takeshi; Tachibana, Taro; Tamagaki, Seizo; Shinkai, Seiji.

In: Journal of Controlled Release, Vol. 103, No. 1, 02.03.2005, p. 199-207.

Research output: Contribution to journalArticle

Nagasaki, Takeshi ; Kawazu, Takeshi ; Tachibana, Taro ; Tamagaki, Seizo ; Shinkai, Seiji. / Enhanced nuclear import and transfection efficiency of plasmid DNA using streptavidin-fused importin-β. In: Journal of Controlled Release. 2005 ; Vol. 103, No. 1. pp. 199-207.
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