Enhanced processivity of Dnmt1 by monoubiquitinated histone H3

Yuichi Mishima; Laura Brueckner; Saori Takahashi; Toru Kawakami; Junji Otani; Akira Shinohara; Kohei Takeshita; Ronald Garingalao Garvilles; Mikio Watanabe; Norio Sakai; Hideyuki Takeshima; Charlotte Nachtegael; Atsuya Nishiyama; Makoto Nakanishi; Kyohei Arita; Kinichi Nakashima; Hironobu Hojo; Isao Suetake

doi:10.1111/gtc.12732

Enhanced processivity of Dnmt1 by monoubiquitinated histone H3

Yuichi Mishima, Laura Brueckner, Saori Takahashi, Toru Kawakami, Junji Otani, Akira Shinohara, Kohei Takeshita, Ronald Garingalao Garvilles, Mikio Watanabe, Norio Sakai, Hideyuki Takeshima, Charlotte Nachtegael, Atsuya Nishiyama, Makoto Nakanishi, Kyohei Arita, Kinichi Nakashima, Hironobu Hojo, Isao Suetake

Stem Cell Biology and Medicine

Research output: Contribution to journal › Article

Abstract

DNA methylation controls gene expression, and once established, DNA methylation patterns are faithfully copied during DNA replication by the maintenance DNA methyltransferase Dnmt1. In vivo, Dnmt1 interacts with Uhrf1, which recognizes hemimethylated CpGs. Recently, we reported that Uhrf1-catalyzed K18- and K23-ubiquitinated histone H3 binds to the N-terminal region (the replication focus targeting sequence, RFTS) of Dnmt1 to stimulate its methyltransferase activity. However, it is not yet fully understood how ubiquitinated histone H3 stimulates Dnmt1 activity. Here, we show that monoubiquitinated histone H3 stimulates Dnmt1 activity toward DNA with multiple hemimethylated CpGs but not toward DNA with only a single hemimethylated CpG, suggesting an influence of ubiquitination on the processivity of Dnmt1. The Dnmt1 activity stimulated by monoubiquitinated histone H3 was additively enhanced by the Uhrf1 SRA domain, which also binds to RFTS. Thus, Dnmt1 activity is regulated by catalysis (ubiquitination)-dependent and -independent functions of Uhrf1.

Original language	English
Pages (from-to)	22-32
Number of pages	11
Journal	Genes to Cells
Volume	25
Issue number	1
DOIs	https://doi.org/10.1111/gtc.12732
Publication status	Published - Jan 1 2020

All Science Journal Classification (ASJC) codes

Genetics
Cell Biology

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10.1111/gtc.12732

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Mishima, Y., Brueckner, L., Takahashi, S., Kawakami, T., Otani, J., Shinohara, A., Takeshita, K., Garvilles, R. G., Watanabe, M., Sakai, N., Takeshima, H., Nachtegael, C., Nishiyama, A., Nakanishi, M., Arita, K., Nakashima, K., Hojo, H., & Suetake, I. (2020). Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. Genes to Cells, 25(1), 22-32. https://doi.org/10.1111/gtc.12732

Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. / Mishima, Yuichi; Brueckner, Laura; Takahashi, Saori; Kawakami, Toru; Otani, Junji; Shinohara, Akira; Takeshita, Kohei; Garvilles, Ronald Garingalao; Watanabe, Mikio; Sakai, Norio; Takeshima, Hideyuki; Nachtegael, Charlotte; Nishiyama, Atsuya; Nakanishi, Makoto; Arita, Kyohei; Nakashima, Kinichi; Hojo, Hironobu; Suetake, Isao.

In: Genes to Cells, Vol. 25, No. 1, 01.01.2020, p. 22-32.

Research output: Contribution to journal › Article

Mishima, Y, Brueckner, L, Takahashi, S, Kawakami, T, Otani, J, Shinohara, A, Takeshita, K, Garvilles, RG, Watanabe, M, Sakai, N, Takeshima, H, Nachtegael, C, Nishiyama, A, Nakanishi, M, Arita, K, Nakashima, K, Hojo, H & Suetake, I 2020, 'Enhanced processivity of Dnmt1 by monoubiquitinated histone H3', Genes to Cells, vol. 25, no. 1, pp. 22-32. https://doi.org/10.1111/gtc.12732

Mishima, Yuichi ; Brueckner, Laura ; Takahashi, Saori ; Kawakami, Toru ; Otani, Junji ; Shinohara, Akira ; Takeshita, Kohei ; Garvilles, Ronald Garingalao ; Watanabe, Mikio ; Sakai, Norio ; Takeshima, Hideyuki ; Nachtegael, Charlotte ; Nishiyama, Atsuya ; Nakanishi, Makoto ; Arita, Kyohei ; Nakashima, Kinichi ; Hojo, Hironobu ; Suetake, Isao. / Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. In: Genes to Cells. 2020 ; Vol. 25, No. 1. pp. 22-32.

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abstract = "DNA methylation controls gene expression, and once established, DNA methylation patterns are faithfully copied during DNA replication by the maintenance DNA methyltransferase Dnmt1. In vivo, Dnmt1 interacts with Uhrf1, which recognizes hemimethylated CpGs. Recently, we reported that Uhrf1-catalyzed K18- and K23-ubiquitinated histone H3 binds to the N-terminal region (the replication focus targeting sequence, RFTS) of Dnmt1 to stimulate its methyltransferase activity. However, it is not yet fully understood how ubiquitinated histone H3 stimulates Dnmt1 activity. Here, we show that monoubiquitinated histone H3 stimulates Dnmt1 activity toward DNA with multiple hemimethylated CpGs but not toward DNA with only a single hemimethylated CpG, suggesting an influence of ubiquitination on the processivity of Dnmt1. The Dnmt1 activity stimulated by monoubiquitinated histone H3 was additively enhanced by the Uhrf1 SRA domain, which also binds to RFTS. Thus, Dnmt1 activity is regulated by catalysis (ubiquitination)-dependent and -independent functions of Uhrf1.",

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