Enhanced processivity of Dnmt1 by monoubiquitinated histone H3

Yuichi Mishima, Laura Brueckner, Saori Takahashi, Toru Kawakami, Junji Otani, Akira Shinohara, Kohei Takeshita, Ronald Garingalao Garvilles, Mikio Watanabe, Norio Sakai, Hideyuki Takeshima, Charlotte Nachtegael, Atsuya Nishiyama, Makoto Nakanishi, Kyohei Arita, Kinichi Nakashima, Hironobu Hojo, Isao Suetake

Research output: Contribution to journalArticle

Abstract

DNA methylation controls gene expression, and once established, DNA methylation patterns are faithfully copied during DNA replication by the maintenance DNA methyltransferase Dnmt1. In vivo, Dnmt1 interacts with Uhrf1, which recognizes hemimethylated CpGs. Recently, we reported that Uhrf1-catalyzed K18- and K23-ubiquitinated histone H3 binds to the N-terminal region (the replication focus targeting sequence, RFTS) of Dnmt1 to stimulate its methyltransferase activity. However, it is not yet fully understood how ubiquitinated histone H3 stimulates Dnmt1 activity. Here, we show that monoubiquitinated histone H3 stimulates Dnmt1 activity toward DNA with multiple hemimethylated CpGs but not toward DNA with only a single hemimethylated CpG, suggesting an influence of ubiquitination on the processivity of Dnmt1. The Dnmt1 activity stimulated by monoubiquitinated histone H3 was additively enhanced by the Uhrf1 SRA domain, which also binds to RFTS. Thus, Dnmt1 activity is regulated by catalysis (ubiquitination)-dependent and -independent functions of Uhrf1.

Original languageEnglish
JournalGenes to Cells
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Histones
Ubiquitination
Methyltransferases
DNA Methylation
DNA
Catalysis
DNA Replication
Maintenance
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Mishima, Y., Brueckner, L., Takahashi, S., Kawakami, T., Otani, J., Shinohara, A., ... Suetake, I. (Accepted/In press). Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. Genes to Cells. https://doi.org/10.1111/gtc.12732

Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. / Mishima, Yuichi; Brueckner, Laura; Takahashi, Saori; Kawakami, Toru; Otani, Junji; Shinohara, Akira; Takeshita, Kohei; Garvilles, Ronald Garingalao; Watanabe, Mikio; Sakai, Norio; Takeshima, Hideyuki; Nachtegael, Charlotte; Nishiyama, Atsuya; Nakanishi, Makoto; Arita, Kyohei; Nakashima, Kinichi; Hojo, Hironobu; Suetake, Isao.

In: Genes to Cells, 01.01.2019.

Research output: Contribution to journalArticle

Mishima, Y, Brueckner, L, Takahashi, S, Kawakami, T, Otani, J, Shinohara, A, Takeshita, K, Garvilles, RG, Watanabe, M, Sakai, N, Takeshima, H, Nachtegael, C, Nishiyama, A, Nakanishi, M, Arita, K, Nakashima, K, Hojo, H & Suetake, I 2019, 'Enhanced processivity of Dnmt1 by monoubiquitinated histone H3', Genes to Cells. https://doi.org/10.1111/gtc.12732
Mishima Y, Brueckner L, Takahashi S, Kawakami T, Otani J, Shinohara A et al. Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. Genes to Cells. 2019 Jan 1. https://doi.org/10.1111/gtc.12732
Mishima, Yuichi ; Brueckner, Laura ; Takahashi, Saori ; Kawakami, Toru ; Otani, Junji ; Shinohara, Akira ; Takeshita, Kohei ; Garvilles, Ronald Garingalao ; Watanabe, Mikio ; Sakai, Norio ; Takeshima, Hideyuki ; Nachtegael, Charlotte ; Nishiyama, Atsuya ; Nakanishi, Makoto ; Arita, Kyohei ; Nakashima, Kinichi ; Hojo, Hironobu ; Suetake, Isao. / Enhanced processivity of Dnmt1 by monoubiquitinated histone H3. In: Genes to Cells. 2019.
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