TY - JOUR
T1 - Enhanced sensitivity of a surface-plasmon-resonance (SPR) sensor for 2,4-D by controlled functionalization of self-assembled monolayer-based immunosensor chip
AU - Kim, Sook Jin
AU - Gobi, K. Vengatajalabathy
AU - Tanaka, Hiroyuki
AU - Shoyama, Yukihiro
AU - Miura, Norio
PY - 2006/10/5
Y1 - 2006/10/5
N2 - A highly sensitive SPR immunosensor has been fabricated by covalent binding of a protein conjugate of 2,4-D (2,4-D-BSA) on SAM of 3-mercaptopropionic acid. With indirect inhibition immunoassay, it demonstrated a low detection limit of 0.1 ppb 2,4-D. Taking advantage of the combination of the SAM technique and immobilization of 2,4-D-BSA at controlled surface coverage, a remarkable enhancement of the low detection limit by 10-fold to 10 ppt with no use of any labels was demonstrated, while keeping the response time constant at 4 min.
AB - A highly sensitive SPR immunosensor has been fabricated by covalent binding of a protein conjugate of 2,4-D (2,4-D-BSA) on SAM of 3-mercaptopropionic acid. With indirect inhibition immunoassay, it demonstrated a low detection limit of 0.1 ppb 2,4-D. Taking advantage of the combination of the SAM technique and immobilization of 2,4-D-BSA at controlled surface coverage, a remarkable enhancement of the low detection limit by 10-fold to 10 ppt with no use of any labels was demonstrated, while keeping the response time constant at 4 min.
UR - http://www.scopus.com/inward/record.url?scp=33846034082&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846034082&partnerID=8YFLogxK
U2 - 10.1246/cl.2006.1132
DO - 10.1246/cl.2006.1132
M3 - Article
AN - SCOPUS:33846034082
SN - 0366-7022
VL - 35
SP - 1132
EP - 1133
JO - Chemistry Letters
JF - Chemistry Letters
IS - 10
ER -