Enhanced sensitivity of a surface-plasmon-resonance (SPR) sensor for 2,4-D by controlled functionalization of self-assembled monolayer-based immunosensor chip

Sook Jin Kim, K. Vengatajalabathy Gobi, Hiroyuki Tanaka, Yukihiro Shoyama, Norio Miura

Research output: Contribution to journalArticle

18 Citations (Scopus)


A highly sensitive SPR immunosensor has been fabricated by covalent binding of a protein conjugate of 2,4-D (2,4-D-BSA) on SAM of 3-mercaptopropionic acid. With indirect inhibition immunoassay, it demonstrated a low detection limit of 0.1 ppb 2,4-D. Taking advantage of the combination of the SAM technique and immobilization of 2,4-D-BSA at controlled surface coverage, a remarkable enhancement of the low detection limit by 10-fold to 10 ppt with no use of any labels was demonstrated, while keeping the response time constant at 4 min.

Original languageEnglish
Pages (from-to)1132-1133
Number of pages2
JournalChemistry Letters
Issue number10
Publication statusPublished - Oct 5 2006


All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this