Enhanced stomatal conductance by a spontaneous Arabidopsis tetraploid, Me-0, results from increased stomatal size and greater stomatal aperture

Keina Monda, Hiromitsu Araki, Satoru Kuhara, Genki Ishigaki, Ryo Akashi, Juntaro Negi, Mikiko Kojima, Hitoshi Sakakibara, Sho Takahashi, Mimi Hashimoto-Sugimoto, Nobuharu Goto, Koh Iba

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The rate of gas exchange in plants is regulated mainly by stomatal size and density. Generally, higher densities of smaller stomata are advantageous for gas exchange; however, it is unclear what the effect of an extraordinary change in stomatal size might have on a plant’s gas-exchange capacity. We investigated the stomatal responses to CO2 concentration changes among 374 Arabidopsis (Arabidopsis thaliana) ecotypes and discovered that Mechtshausen (Me-0), a natural tetraploid ecotype, has significantly larger stomata and can achieve a high stomatal conductance. We surmised that the cause of the increased stomatal conductance is tetraploidization; however, the stomatal conductance of another tetraploid accession, tetraploid Columbia (Col), was not as high as that in Me-0. One difference between these two accessions was the size of their stomatal apertures. Analyses of abscisic acid sensitivity, ion balance, and gene expression profiles suggested that physiological or genetic factors restrict the stomatal opening in tetraploid Col but not in Me-0. Our results show that Me-0 overcomes the handicap of stomatal opening that is typical for tetraploids and achieves higher stomatal conductance compared with the closely related tetraploid Col on account of larger stomatal apertures. This study provides evidence for whether larger stomatal size in tetraploids of higher plants can improve stomatal conductance.

Original languageEnglish
Pages (from-to)1435-1444
Number of pages10
JournalPlant physiology
Volume170
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Tetraploidy
Arabidopsis
tetraploidy
stomatal conductance
Ecotype
gas exchange
Gases
ecotypes
stomata
Abscisic Acid
Transcriptome
abscisic acid
Arabidopsis thaliana
Ions
ions
gene expression

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Enhanced stomatal conductance by a spontaneous Arabidopsis tetraploid, Me-0, results from increased stomatal size and greater stomatal aperture. / Monda, Keina; Araki, Hiromitsu; Kuhara, Satoru; Ishigaki, Genki; Akashi, Ryo; Negi, Juntaro; Kojima, Mikiko; Sakakibara, Hitoshi; Takahashi, Sho; Hashimoto-Sugimoto, Mimi; Goto, Nobuharu; Iba, Koh.

In: Plant physiology, Vol. 170, No. 3, 01.03.2016, p. 1435-1444.

Research output: Contribution to journalArticle

Monda, Keina ; Araki, Hiromitsu ; Kuhara, Satoru ; Ishigaki, Genki ; Akashi, Ryo ; Negi, Juntaro ; Kojima, Mikiko ; Sakakibara, Hitoshi ; Takahashi, Sho ; Hashimoto-Sugimoto, Mimi ; Goto, Nobuharu ; Iba, Koh. / Enhanced stomatal conductance by a spontaneous Arabidopsis tetraploid, Me-0, results from increased stomatal size and greater stomatal aperture. In: Plant physiology. 2016 ; Vol. 170, No. 3. pp. 1435-1444.
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