Enhanced visualization of small peptides absorbed in rat small intestine by phytic-acid-aided matrix-assisted laser desorption/ionization-imaging mass spectrometry

Seong Min Hong, Mitsuru Tanaka, Saori Yoshii, Yoshinori Mine, Toshiro Matsui

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Enhanced visualization of small peptides absorbed through a rat intestinal membrane was achieved by matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-IMS) with the aid of phytic acid as a matrix additive. Penetrants through intestinal peptide transporter 1, i.e., glycyl-sarcosine (Gly-Sar, 147.1 m/z) and antihypertensive dipeptide, Val-Tyr (281.2 m/z), were chosen for MALDI-IMS. The signal-to-noise (S/N) ratios of dipeptides Gly-Sar and Val-Tyr were seen to increase by 2.4- and 8.0-fold, respectively, when using a 2′,4′,6′-trihydroxyacetophenone (THAP) matrix containing 5.0 mM phytic acid, instead of the THAP matrix alone. Owing to the phytic-acid-aided MALDI-IMS method, Gly-Sar and Val-Tyr absorbed in the rat intestinal membrane were successfully visualized. The proposed imaging method also provided useful information on intestinal peptide absorption; to some extent, Val-Tyr was rapidly hydrolyzed to Tyr by peptidases located at the intestinal microvillus during the absorption process. In conclusion, the strongly acidic additive, phytic acid, is beneficial for enhancing the visualization of small peptides using MALDI-IMS, owing to the suppression of ionization-interfering salts in the tissue.

Original languageEnglish
Pages (from-to)10033-10039
Number of pages7
JournalAnalytical Chemistry
Volume85
Issue number21
DOIs
Publication statusPublished - Nov 5 2013

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Phytic Acid
Ionization
Mass spectrometry
Rats
Desorption
Visualization
Imaging techniques
Peptides
Lasers
Dipeptides
Sarcosine
Membranes
Antihypertensive Agents
Signal to noise ratio
Peptide Hydrolases
Salts
Tissue
glycylsarcosine

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Enhanced visualization of small peptides absorbed in rat small intestine by phytic-acid-aided matrix-assisted laser desorption/ionization-imaging mass spectrometry. / Hong, Seong Min; Tanaka, Mitsuru; Yoshii, Saori; Mine, Yoshinori; Matsui, Toshiro.

In: Analytical Chemistry, Vol. 85, No. 21, 05.11.2013, p. 10033-10039.

Research output: Contribution to journalArticle

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