Enhancement by zinc of ATP-evoked dopamine release from rat pheochromocytoma PC12 cells

Schuichi Koizumi, Makoto Ikeda, Kaori Inoue, Ken Nakazawa, Kazuhide Inoue

Research output: Contribution to journalArticle

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Abstract

The effects of zinc (Zn 2+ ) on ATP-evoked dopamine release was investigated in rat pheochromocytoma PC12 cells. Zn 2+ potentiated the dopamine release evoked by 30 μM ATP in a concentration-dependent manner over a concentration range from 3 to 300 μM. High concentration of Zn 2+ (>1 mM) inhibited the release. Zn 2+ (10 μM) shifted the concentration-response curve of the ATP-evoked dopamine release to the left without affecting the maximal response. The dopamine release evoked by 40 mM KCl was not affected by Zn 2+ (1-100 μM), whereas high concentration of Zn 2+ (>300 μM) attenuated the response. The dopamine release evoked by 30 μM ATP in the presence of 10 μM Zn 2+ were suppressed by suramin (30 μM), an antagonist to P 2 -purinoceptors, to an extent similar to that in the absence of Zn 2+ . Zn 2+ (1-100 μM) enhanced the ATP-evoked increase in intracellular Ca 2+ concentration ([Ca]i) in the cells. The Ca 2+ responses to ATP in the presence and absence of Zn 2+ were abolished by external Ca 2+ -depletion. Under whole-cell voltage-clamp, Zn 2+ (10 μM) augmented by two-fold the peak amplitude of an inward current evoked by 30 μM ATP. Taken together, it is suggested that Zn 2+ enhances the ATP-evoked dopamine release by increasing sensitivity to ATP. The enhancement may be due to the augmentation of ATP-gated Ca 2+ -influx, but not due to modulation of cellular machinery downstream to [Ca]i rise. The enhancement of the ATP-mediated responses may underlie modulation by Zn 2+ of physiological functions in various types of neuronal cells.

Original languageEnglish
Pages (from-to)75-82
Number of pages8
JournalBrain Research
Volume673
Issue number1
DOIs
Publication statusPublished - Feb 27 1995
Externally publishedYes

Fingerprint

PC12 Cells
Pheochromocytoma
Zinc
Dopamine
Adenosine Triphosphate
Purinergic Receptors
Suramin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Enhancement by zinc of ATP-evoked dopamine release from rat pheochromocytoma PC12 cells. / Koizumi, Schuichi; Ikeda, Makoto; Inoue, Kaori; Nakazawa, Ken; Inoue, Kazuhide.

In: Brain Research, Vol. 673, No. 1, 27.02.1995, p. 75-82.

Research output: Contribution to journalArticle

Koizumi, Schuichi ; Ikeda, Makoto ; Inoue, Kaori ; Nakazawa, Ken ; Inoue, Kazuhide. / Enhancement by zinc of ATP-evoked dopamine release from rat pheochromocytoma PC12 cells. In: Brain Research. 1995 ; Vol. 673, No. 1. pp. 75-82.
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