Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs

Jin Zheng, Yukihiro Tashiro, Tao Zhao, Qunhui Wang, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eucalyptus has been previously suggested as a potential substrate for acetone-butanol-ethanol (ABE) production without nutrient supplementation; however, incomplete sugar consumption has prevented improvement of ABE production. Cellulase loading with 35 FPU g−1 was first optimized in terms of high hydrolysis efficiency (95%). However, only 0.43 g L−1 ABE production and 3.44% glucose consumption rate were achieved. To improve ABE production from eucalyptus hydrolysate, supplementation of 6 nutrients in common tryptone-yeast extract (TY) medium were investigated by statistical approaches. Three nutrients including yeast extract, tryptone, and FeSO4·7H2O were screened as significant nutrients for ABE production. Subsequently, use of a modified TY medium (MTY medium: yeast extract 3.04 g L−1, tryptone 7.64 g L−1, FeSO4·7H2O 15.3 mg L−1), which was subsequently predicted by Plackett-Burman and Box-Behnken designs to stimulate ABE production, resulted in ca. 40-fold increase in ABE concentration (16.9 g L−1) and a glucose consumption rate of 100%. We first examined previously uninvestigated nutrition combinations using Plackett-Burman and Box-Behnken designs for high ABE production from eucalyptus hydrolysate. This study shows that statistical method would be a powerful tool for the optimization and enhancement of ABE production from eucalyptus hydrolysate.

Original languageEnglish
Pages (from-to)580-586
Number of pages7
JournalRenewable Energy
Volume113
DOIs
Publication statusPublished - Jan 1 2017

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Butenes
Acetone
Design of experiments
Fermentation
Nutrients
Ethanol
Yeast
Glucose
Nutrition
Sugars
Hydrolysis
Statistical methods
Substrates

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment

Cite this

Enhancement of acetone-butanol-ethanol fermentation from eucalyptus hydrolysate with optimized nutrient supplementation through statistical experimental designs. / Zheng, Jin; Tashiro, Yukihiro; Zhao, Tao; Wang, Qunhui; Sakai, Kenji; Sonomoto, Kenji.

In: Renewable Energy, Vol. 113, 01.01.2017, p. 580-586.

Research output: Contribution to journalArticle

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