Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus

Takumi Abe, Norifumi Miyake, Yasuyuki Nishijima, Ryosuke Fujita, Ken Sahara, Shin Ichiro Asano, Hisanori Bando

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We happened to discover that the cauliflower mosaic virus (CaMV) 35S promoter inserted into a recombinant Autographa californica multicapsid nucleopolyhedrovirus (rAcMNPV) was strongly activated during the replication of the recombinant virus in Spodoptera frugiperda (Sf9) cells. The expression of the luciferase gene from the 35S promoter in rAcMNPV was remarkably increased late in infection and was resistant to alpha-amanitin treatment. Primer extension indicated that transcriptional initiation from the 35S promoter in Sf9 cells occurred within one of the two baculoviral late promoter TAAG motifs located in the vicinity of the transcription start site in plant cells. These observations suggested that the CaMV 35S promoter served as a transcription start site for AcMNPV-induced RNA polymerase.

Original languageEnglish
Pages (from-to)38-41
Number of pages4
JournalVirus Research
Volume112
Issue number1-2
DOIs
Publication statusPublished - Sep 1 2005
Externally publishedYes

Fingerprint

Caulimovirus
Nucleopolyhedrovirus
Sf9 Cells
Baculoviridae
Transcription Initiation Site
Insects
Alpha-Amanitin
Spodoptera
Plant Cells
DNA-Directed RNA Polymerases
Virus Replication
Luciferases
Gene Expression
Infection

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus. / Abe, Takumi; Miyake, Norifumi; Nishijima, Yasuyuki; Fujita, Ryosuke; Sahara, Ken; Asano, Shin Ichiro; Bando, Hisanori.

In: Virus Research, Vol. 112, No. 1-2, 01.09.2005, p. 38-41.

Research output: Contribution to journalArticle

Abe, Takumi ; Miyake, Norifumi ; Nishijima, Yasuyuki ; Fujita, Ryosuke ; Sahara, Ken ; Asano, Shin Ichiro ; Bando, Hisanori. / Enhancement of cauliflower mosaic virus 35S promoter in insect cells infected with baculovirus. In: Virus Research. 2005 ; Vol. 112, No. 1-2. pp. 38-41.
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