Enhancement of EPR effect by using NO releasing nano-liposome

EPR effect is one of the most versatile and useful effect to deliver nano-medicine to solid tumor. However, the extravasation ability sometimes seems to be suppressed in some cases. Thus, enhancement of EPR effect will be useful to ensure and augment of therapeutic ability of nano-medicine. Here a liposome in which spontaneous niric oxide releaser was encapsulated is introduced as an enhancement system of EPR effect. The liposome releases nitric oxide with 35h of half-life time. When the liposome was injected from tail vein of CT26 tumor bearing mice, the liposome accumulation in the tumor increased twice comparing with empty liposome without any change of the liposome accumulation in other organs. Co-injection of the liposome and Doxil also enhanced the therapeutic effect of Doxil itself. The system will contribute cancer chemotherapy using nano-medicine.