TY - JOUR
T1 - Enhancement of immunoglobulin a production in peyer's patches by oral administration of a traditional chinese medicine, xiao-chai-hu-tang (shosaiko-to)
AU - Tauchi, Yoshihiko
AU - Kawakita, Takuva
AU - Saito, Yuji
AU - Suzuki, Akira
AU - Yamada, Akira
AU - Yoshikai, Yasunobu
AU - Nomoto, Kikuo
PY - 1993
Y1 - 1993
N2 - The Peyer's patches contain a large number of precursor cells committed to the production of immunoglobulin A (IgA) and play an important role in IgA response in the mucosal immune system. We investigated the induction of IgA producing cells in Peyer's patches by plaque forming cell assay after oral administration of a traditional Chinese herbal medicine, Xiao-chai-hu-tang (Japanese name: Shosaiko-to). The number of total IgA producing cells in the Peyer's patches detected by the protein-A plaque assay was increased about two-fold by Shosaiko-to administration and the numbers of both anti-SRBC and anti-HRBC IgA producing cells were also increased by such a treatment. On the other hand, when SRBC alone were administered orally, only the number of anti-SRBC IgA producing cells was increased. Further, we examined T-cell subpopulations in the gut-associated lymphoid tissues after oral administration of Shosaiko-to by flowcytometry. Marked alternations in T cell subpopulations were not detected in the Peyer's patches, though TcR TcR γdelta;+T-cells in the intraepithelial lymphocytes and Thy 1.2-TcRαbeta;+T cells in the mesenteric lymph nodes were slight increased. These results showed that orally administered Shosaiko-to acts as a polyclonal B-cell activator which induces IgA production in the mucosal immune system.
AB - The Peyer's patches contain a large number of precursor cells committed to the production of immunoglobulin A (IgA) and play an important role in IgA response in the mucosal immune system. We investigated the induction of IgA producing cells in Peyer's patches by plaque forming cell assay after oral administration of a traditional Chinese herbal medicine, Xiao-chai-hu-tang (Japanese name: Shosaiko-to). The number of total IgA producing cells in the Peyer's patches detected by the protein-A plaque assay was increased about two-fold by Shosaiko-to administration and the numbers of both anti-SRBC and anti-HRBC IgA producing cells were also increased by such a treatment. On the other hand, when SRBC alone were administered orally, only the number of anti-SRBC IgA producing cells was increased. Further, we examined T-cell subpopulations in the gut-associated lymphoid tissues after oral administration of Shosaiko-to by flowcytometry. Marked alternations in T cell subpopulations were not detected in the Peyer's patches, though TcR TcR γdelta;+T-cells in the intraepithelial lymphocytes and Thy 1.2-TcRαbeta;+T cells in the mesenteric lymph nodes were slight increased. These results showed that orally administered Shosaiko-to acts as a polyclonal B-cell activator which induces IgA production in the mucosal immune system.
UR - http://www.scopus.com/inward/record.url?scp=0027173315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027173315&partnerID=8YFLogxK
U2 - 10.3109/08923979309025998
DO - 10.3109/08923979309025998
M3 - Article
C2 - 8349952
AN - SCOPUS:0027173315
VL - 15
SP - 251
EP - 272
JO - Immunopharmacology and Immunotoxicology
JF - Immunopharmacology and Immunotoxicology
SN - 0892-3973
IS - 2-3
ER -