Enhancement of NMDA receptor-mediated synaptic potential evoked in rat medial-amygdala neuron following olfactory bulbectomy

Hiroshi Nakanishi, Kiyoharu Ukai, Terutake Nakagawa, Shigenori Watanabe, Osamu Kamata, Kenji Yamamoto

Research output: Contribution to journalArticle

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Abstract

Synaptic potentials evoked in the medial amygdala (m-AMG) neurons were studied in in vitro slice preparations obtained from normal and olfactory bulbectomized rats. Local stimulation induced a sequence of responses: a fast EPSP, a fast IPSP and a slow EPSP. The fast EPSP was suppressed by kynurenic acid (KYN) at a concentration of 1 mM but not by 3-[(±)-2-carboxypiperazin-4-yl-]-propyl-1-phosphonic acid (CPP) at a concentrations up to 20 μM. The slow EPSP was reversibly blocked by both KYN (1 mM) and CPP (5-10 μM). Addition of bicuculline methiodide (50 μM) to the bath suppressed the fast IPSP and augmented both the fast and slow EPSPs leading to burst discharges. In a small population of m-AMG neurons, the slow EPSP was followed by a slow IPSP. Theslow IPSP was suppressed by phaclofen (500 μM) but not by bicuculline methiodide (up to 100 μM). In slice preparations obtained from olfactory bulbectomized rats, local stimulation evoked burst discharges, which were similar to those observed when bicuculline methiodide was applied to slice preparations obtained from normal rats. These results suggest that GABAA receptor mediating fast IPSP and N-methyl-D-aspartate (NMDA) receptors mediating slow EPSP regulate activities of m-AMG neurons and that the enhancement of NMDA receptor mediating slow EPSP is responsible for the hyperexcitability of m-AMG neurons following olfactory bulbectomy.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalBrain Research
Volume532
Issue number1-2
DOIs
Publication statusPublished - Nov 5 1990

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Synaptic Potentials
Excitatory Postsynaptic Potentials
Amygdala
N-Methyl-D-Aspartate Receptors
Inhibitory Postsynaptic Potentials
Neurons
Kynurenic Acid
GABA-A Receptors
Baths

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Enhancement of NMDA receptor-mediated synaptic potential evoked in rat medial-amygdala neuron following olfactory bulbectomy. / Nakanishi, Hiroshi; Ukai, Kiyoharu; Nakagawa, Terutake; Watanabe, Shigenori; Kamata, Osamu; Yamamoto, Kenji.

In: Brain Research, Vol. 532, No. 1-2, 05.11.1990, p. 69-75.

Research output: Contribution to journalArticle

Nakanishi, Hiroshi ; Ukai, Kiyoharu ; Nakagawa, Terutake ; Watanabe, Shigenori ; Kamata, Osamu ; Yamamoto, Kenji. / Enhancement of NMDA receptor-mediated synaptic potential evoked in rat medial-amygdala neuron following olfactory bulbectomy. In: Brain Research. 1990 ; Vol. 532, No. 1-2. pp. 69-75.
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