Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles

Kosei Endo, Takahisa Anada, Masumi Yamada, Minoru Seki, Keiichi Sasaki, Osamu Suzuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study investigated whether alginate (Alg) hydrogel microbeads have a role in maintaining mouse bone marrow stromal ST-2 cells and release the cells after being stimulated by synthetic octacalcium phosphate (OCP), which is a mineral crystal capable of stimulating osteoblastic differentiation during a conversion process to hydroxyapatite (HA). The ST-2 cell suspension in the alginate solution, which contained various concentrations of OCP granules with diameters less than 53 μm, was extruded drop-wise into a stirred gelation solution containing BaCl2 using an encapsulator with nitrogen gas stream. The Alg-microbeads (Alg/OCP • ST-2 microbeads) that were generated, which had a diameter of approximately 400 μm, were incubated for up to 14 d and then assessed for osteoblastic differentiation. Alg-microbeads with cells were also incubated to identify the possible conversion from OCP to HA. Osteoblast differentiation markers in ST-2 cells, alkaline phosphatase (ALP) and collagen type I, were up-regulated in the presence of higher amounts of OCP. X-ray diffraction analysis and Fourier transform infrared spectroscopy confirmed that the OCP tended to convert to HA over time, suggesting that the OCP in Alg-microbeads interacts three-dimensionally with ST-2 cells and stimulates its osteoblastic differentiation. The release of ST-2 cells from the microbeads was also estimated. ST-2 cells were identified outside of the microbeads, although the cell number tended to decrease with increasing OCP. These results suggest that Alg/OCP microbeads could be used as a vehicle to activate osteoblastic cells and deliver them to sites where bone regeneration is needed.

Original languageEnglish
Article number065019
JournalBiomedical Materials (Bristol)
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 14 2015
Externally publishedYes

Fingerprint

Alginate
Particles (particulate matter)
Phosphates
Gels
Durapatite
Hydroxyapatite
Bone
alginic acid
octacalcium phosphate
Hydrogel
Differentiation Antigens
Osteoblasts
Phosphatases
Gelation
Collagen Type I
Collagen
Hydrogels
X ray diffraction analysis
Fourier transform infrared spectroscopy
Minerals

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles. / Endo, Kosei; Anada, Takahisa; Yamada, Masumi; Seki, Minoru; Sasaki, Keiichi; Suzuki, Osamu.

In: Biomedical Materials (Bristol), Vol. 10, No. 6, 065019, 14.12.2015.

Research output: Contribution to journalArticle

Endo, Kosei ; Anada, Takahisa ; Yamada, Masumi ; Seki, Minoru ; Sasaki, Keiichi ; Suzuki, Osamu. / Enhancement of osteoblastic differentiation in alginate gel beads with bioactive octacalcium phosphate particles. In: Biomedical Materials (Bristol). 2015 ; Vol. 10, No. 6.
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