TY - JOUR
T1 - Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2
AU - Furutani, Takashi
AU - Hazeyama, Kohsuke
AU - Ueda, Toshifumi
AU - Tomita, Satoko
AU - Imai, Takayoshi
AU - Nakashima, Takashi
AU - Kakuta, Yoshimitsu
AU - Kimura, Makoto
N1 - Funding Information:
This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan to M.K. [grant number 22380062].
PY - 2014
Y1 - 2014
N2 - We analyzed modes of action of ribonuclease P (RNase P) proteins, C5 in Escherichia coli and Rpr2 in Saccharomyces cerevisiae, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer-based assays revealed that RNA annealing and strand displacement activities found in archaeal RNase P proteins are prevalent in eubacterial (C5) and eukaryotic (Rpr2) RNase P proteins.
AB - We analyzed modes of action of ribonuclease P (RNase P) proteins, C5 in Escherichia coli and Rpr2 in Saccharomyces cerevisiae, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer-based assays revealed that RNA annealing and strand displacement activities found in archaeal RNase P proteins are prevalent in eubacterial (C5) and eukaryotic (Rpr2) RNase P proteins.
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U2 - 10.1080/09168451.2014.925780
DO - 10.1080/09168451.2014.925780
M3 - Article
C2 - 25273134
AN - SCOPUS:84930746247
VL - 78
SP - 1700
EP - 1702
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -