Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2

Takashi Furutani, Kohsuke Hazeyama, Toshifumi Ueda, Satoko Tomita, Takayoshi Imai, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journal › Article

Abstract

We analyzed modes of action of ribonuclease P (RNase P) proteins, C5 in Escherichia coli and Rpr2 in Saccharomyces cerevisiae, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer-based assays revealed that RNA annealing and strand displacement activities found in archaeal RNase P proteins are prevalent in eubacterial (C5) and eukaryotic (Rpr2) RNase P proteins.

Original languageEnglish
Pages (from-to)1700-1702
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number10
DOIs
Publication statusPublished - Jan 1 2014

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Ribonuclease P
Fungal Proteins
Escherichia coli Proteins
RNA
Annealing
Oligoribonucleotides
Fluorescence Resonance Energy Transfer
Proteins
Yeast
Escherichia coli
Saccharomyces cerevisiae
Assays
Fluorescence

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2. / Furutani, Takashi; Hazeyama, Kohsuke; Ueda, Toshifumi; Tomita, Satoko; Imai, Takayoshi; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto.

In: Bioscience, Biotechnology and Biochemistry, Vol. 78, No. 10, 01.01.2014, p. 1700-1702.

Research output: Contribution to journal › Article

Furutani, T, Hazeyama, K, Ueda, T, Tomita, S, Imai, T, Nakashima, T, Kakuta, Y & Kimura, M 2014, 'Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2', Bioscience, Biotechnology and Biochemistry, vol. 78, no. 10, pp. 1700-1702. https://doi.org/10.1080/09168451.2014.925780
Furutani T, Hazeyama K, Ueda T, Tomita S, Imai T, Nakashima T et al. Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2. Bioscience, Biotechnology and Biochemistry. 2014 Jan 1;78(10):1700-1702. https://doi.org/10.1080/09168451.2014.925780
Furutani, Takashi ; Hazeyama, Kohsuke ; Ueda, Toshifumi ; Tomita, Satoko ; Imai, Takayoshi ; Nakashima, Takashi ; Kakuta, Yoshimitsu ; Kimura, Makoto. / Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2. In: Bioscience, Biotechnology and Biochemistry. 2014 ; Vol. 78, No. 10. pp. 1700-1702.
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