Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2

Takashi Furutani, Kohsuke Hazeyama, Toshifumi Ueda, Satoko Tomita, Takayoshi Imai, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

Research output: Contribution to journalArticle

Abstract

We analyzed modes of action of ribonuclease P (RNase P) proteins, C5 in Escherichia coli and Rpr2 in Saccharomyces cerevisiae, using a pair of complementary fluorescence-labeled oligoribonucleotides. Fluorescence resonance energy transfer-based assays revealed that RNA annealing and strand displacement activities found in archaeal RNase P proteins are prevalent in eubacterial (C5) and eukaryotic (Rpr2) RNase P proteins.

Original languageEnglish
Pages (from-to)1700-1702
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume78
Issue number10
DOIs
Publication statusPublished - 2014

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Enhancement of RNA annealing and strand displacement found in archaeal ribonuclease P proteins is conserved in Escherichia coli protein C5 and yeast protein Rpr2'. Together they form a unique fingerprint.

  • Cite this