Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays

Masateru Ito, Fumio Nakamura, Akira Baba, Kaoru Tamada, Hirobumi Ushijima, King Hang Aaron Lau, Abhijit Manna, Wolfgang Knoll

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have developed a new methodology to fabricate high-resolution DNA microarrays (2500 dots/cm2) in combination with microcontact printing (μCP) and surface plasmon resonance (SPR) imaging. A novel COOHterminated PEG-disulfide with para-carborane (p-carborane) was synthesized as an initiator chemically bound to a gold substrate for surface coupling reactions with NH 2-terminated DNA. The hybridization between target DNA immobilized on gold nanoparticles and probe DNA arrayed on flat gold substrates was successfully demonstrated by SPR imaging, where nonspecific adsorption was not observed at the array background. The gold nanoparticles on the array give quite high contrast even at low surface coverage with gold nanoparticles (∼10%) by the enhancement effect of optical signals based on nanoscale phenomena. This enhancement effect can be well described by the simulation based on the Maxwell-Garnett theory for the effective dielectric constants and Fresnel's equation.

Original languageEnglish
Pages (from-to)11653-11662
Number of pages10
JournalJournal of Physical Chemistry C
Volume111
Issue number31
DOIs
Publication statusPublished - Aug 9 2007
Externally publishedYes

Fingerprint

Surface plasmon resonance
Microarrays
surface plasmon resonance
Gold
DNA
deoxyribonucleic acid
gold
Nanoparticles
nanoparticles
augmentation
Immobilized Nucleic Acids
carborane
Imaging techniques
DNA Probes
disulfides
Substrates
initiators
printing
Disulfides
Polyethylene glycols

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Energy(all)
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films

Cite this

Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays. / Ito, Masateru; Nakamura, Fumio; Baba, Akira; Tamada, Kaoru; Ushijima, Hirobumi; Lau, King Hang Aaron; Manna, Abhijit; Knoll, Wolfgang.

In: Journal of Physical Chemistry C, Vol. 111, No. 31, 09.08.2007, p. 11653-11662.

Research output: Contribution to journalArticle

Ito, M, Nakamura, F, Baba, A, Tamada, K, Ushijima, H, Lau, KHA, Manna, A & Knoll, W 2007, 'Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays', Journal of Physical Chemistry C, vol. 111, no. 31, pp. 11653-11662. https://doi.org/10.1021/jp070524m
Ito, Masateru ; Nakamura, Fumio ; Baba, Akira ; Tamada, Kaoru ; Ushijima, Hirobumi ; Lau, King Hang Aaron ; Manna, Abhijit ; Knoll, Wolfgang. / Enhancement of surface plasmon resonance signals by gold nanoparticles on high-density DNA microarrays. In: Journal of Physical Chemistry C. 2007 ; Vol. 111, No. 31. pp. 11653-11662.
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