Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium

Masami Mizu, Kazuya Koumoto, Takahisa Anada, Ryouji Karinaga, Taro Kimura, Takeshi Nagasaki, Seiji Shinkai, Kazuo Sakurai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Schizophyllan is a β-(1 → 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-phosphodiester-polynucleotide is added to the schizophyllan/DMSO solution and, subsequently, DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. In this paper, we report that phosphorothioate oligonucleotides can form a complex with s-SPG in the same manner as phosphodiester oligonucleotides. We carried out an in vitro antisense assay combining melanoma cell lines and a phosphorothioate antisense oligonucleotide (AS ODN) to depress c-myb mRNA. We found that the AS ODN bound to the complex reduces cell growth more efficiently than that of naked AS ODN by preventing the AS ODN from binding to albumin in the culture medium and being hydrolyzed.

Original languageEnglish
Pages (from-to)1101-1110
Number of pages10
JournalBulletin of the Chemical Society of Japan
Volume77
Issue number6
DOIs
Publication statusPublished - Jun 1 2004

Fingerprint

Sizofiran
Polynucleotides
Antisense Oligonucleotides
Dimethyl Sulfoxide
Polysaccharides
Culture Media
Phosphorothioate Oligonucleotides
Proteins
Water
Cell growth
Oligonucleotides
Albumins
Assays
Cells
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium. / Mizu, Masami; Koumoto, Kazuya; Anada, Takahisa; Karinaga, Ryouji; Kimura, Taro; Nagasaki, Takeshi; Shinkai, Seiji; Sakurai, Kazuo.

In: Bulletin of the Chemical Society of Japan, Vol. 77, No. 6, 01.06.2004, p. 1101-1110.

Research output: Contribution to journalArticle

Mizu, Masami ; Koumoto, Kazuya ; Anada, Takahisa ; Karinaga, Ryouji ; Kimura, Taro ; Nagasaki, Takeshi ; Shinkai, Seiji ; Sakurai, Kazuo. / Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium. In: Bulletin of the Chemical Society of Japan. 2004 ; Vol. 77, No. 6. pp. 1101-1110.
@article{985a611054ae4c899c7eaeb3fcdd1fa4,
title = "Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium",
abstract = "Schizophyllan is a β-(1 → 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-phosphodiester-polynucleotide is added to the schizophyllan/DMSO solution and, subsequently, DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. In this paper, we report that phosphorothioate oligonucleotides can form a complex with s-SPG in the same manner as phosphodiester oligonucleotides. We carried out an in vitro antisense assay combining melanoma cell lines and a phosphorothioate antisense oligonucleotide (AS ODN) to depress c-myb mRNA. We found that the AS ODN bound to the complex reduces cell growth more efficiently than that of naked AS ODN by preventing the AS ODN from binding to albumin in the culture medium and being hydrolyzed.",
author = "Masami Mizu and Kazuya Koumoto and Takahisa Anada and Ryouji Karinaga and Taro Kimura and Takeshi Nagasaki and Seiji Shinkai and Kazuo Sakurai",
year = "2004",
month = "6",
day = "1",
doi = "10.1246/bcsj.77.1101",
language = "English",
volume = "77",
pages = "1101--1110",
journal = "Bulletin of the Chemical Society of Japan",
issn = "0009-2673",
publisher = "The Chemical Society of Japan",
number = "6",

}

TY - JOUR

T1 - Enhancement of the antisense effect of polysaccharide-polynucleotide complexes by preventing the antisense oligonucleotide from binding to proteins in the culture medium

AU - Mizu, Masami

AU - Koumoto, Kazuya

AU - Anada, Takahisa

AU - Karinaga, Ryouji

AU - Kimura, Taro

AU - Nagasaki, Takeshi

AU - Shinkai, Seiji

AU - Sakurai, Kazuo

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Schizophyllan is a β-(1 → 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-phosphodiester-polynucleotide is added to the schizophyllan/DMSO solution and, subsequently, DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. In this paper, we report that phosphorothioate oligonucleotides can form a complex with s-SPG in the same manner as phosphodiester oligonucleotides. We carried out an in vitro antisense assay combining melanoma cell lines and a phosphorothioate antisense oligonucleotide (AS ODN) to depress c-myb mRNA. We found that the AS ODN bound to the complex reduces cell growth more efficiently than that of naked AS ODN by preventing the AS ODN from binding to albumin in the culture medium and being hydrolyzed.

AB - Schizophyllan is a β-(1 → 3)-D-glucan existing as a triple helix in water and as a single chain in dimethyl sulfoxide (DMSO), respectively. As we already reported, when a homo-phosphodiester-polynucleotide is added to the schizophyllan/DMSO solution and, subsequently, DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. In this paper, we report that phosphorothioate oligonucleotides can form a complex with s-SPG in the same manner as phosphodiester oligonucleotides. We carried out an in vitro antisense assay combining melanoma cell lines and a phosphorothioate antisense oligonucleotide (AS ODN) to depress c-myb mRNA. We found that the AS ODN bound to the complex reduces cell growth more efficiently than that of naked AS ODN by preventing the AS ODN from binding to albumin in the culture medium and being hydrolyzed.

UR - http://www.scopus.com/inward/record.url?scp=3042532433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042532433&partnerID=8YFLogxK

U2 - 10.1246/bcsj.77.1101

DO - 10.1246/bcsj.77.1101

M3 - Article

AN - SCOPUS:3042532433

VL - 77

SP - 1101

EP - 1110

JO - Bulletin of the Chemical Society of Japan

JF - Bulletin of the Chemical Society of Japan

SN - 0009-2673

IS - 6

ER -