Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains

Taiki Futagami, Fuyuki Okamoto, Hiroshi Hashimoto, Kotaro Fukuzawa, Koji Fukuzawa, K. H.M.Nazmul Hussain Nazir, Erina Wada, Akiko Suyama, Kaoru Takegawa, Masatoshi Goto, Kanji Nakamura, Kensuke Furukawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A microbial consortium that reductively dechlorinates trichloroethene, cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with methanogenesis was enriched from chloroethene-contaminated soil from Japan. Dechlorination activity was maintained for over 4 years. Using quantitative polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were detected. Their growth and dechlorination activities were classified into two types: one that grows by converting cis-DCE to ethene and the other that grows by converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC reductive dehalogenases were detected. Inhibitors of methanogenesis (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of cis-DCE and of VC respectively. These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides".

Original languageEnglish
Pages (from-to)1268-1274
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number7
DOIs
Publication statusPublished - Aug 1 2011

Fingerprint

Trichloroethylene
Vinyl Chloride
Dechlorination
Microbial Consortia
Methanogens
Denaturing Gradient Gel Electrophoresis
Gene encoding
Polymerase chain reaction
Electrophoresis
rRNA Genes
Sulfates
Bacteria
Japan
Soil
Genes
Gels
1,2-dichloroethylene
Soils
Polymerase Chain Reaction
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Futagami, T., Okamoto, F., Hashimoto, H., Fukuzawa, K., Fukuzawa, K., Nazir, K. H. M. N. H., ... Furukawa, K. (2011). Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains. Bioscience, Biotechnology and Biochemistry, 75(7), 1268-1274. https://doi.org/10.1271/bbb.110028

Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains. / Futagami, Taiki; Okamoto, Fuyuki; Hashimoto, Hiroshi; Fukuzawa, Kotaro; Fukuzawa, Koji; Nazir, K. H.M.Nazmul Hussain; Wada, Erina; Suyama, Akiko; Takegawa, Kaoru; Goto, Masatoshi; Nakamura, Kanji; Furukawa, Kensuke.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 7, 01.08.2011, p. 1268-1274.

Research output: Contribution to journalArticle

Futagami, T, Okamoto, F, Hashimoto, H, Fukuzawa, K, Fukuzawa, K, Nazir, KHMNH, Wada, E, Suyama, A, Takegawa, K, Goto, M, Nakamura, K & Furukawa, K 2011, 'Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains', Bioscience, Biotechnology and Biochemistry, vol. 75, no. 7, pp. 1268-1274. https://doi.org/10.1271/bbb.110028
Futagami, Taiki ; Okamoto, Fuyuki ; Hashimoto, Hiroshi ; Fukuzawa, Kotaro ; Fukuzawa, Koji ; Nazir, K. H.M.Nazmul Hussain ; Wada, Erina ; Suyama, Akiko ; Takegawa, Kaoru ; Goto, Masatoshi ; Nakamura, Kanji ; Furukawa, Kensuke. / Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 7. pp. 1268-1274.
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