TY - JOUR
T1 - Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple "Dehalococcoides" strains
AU - Futagami, Taiki
AU - Okamoto, Fuyuki
AU - Hashimoto, Hiroshi
AU - Fukuzawa, Kotaro
AU - Fukuzawa, Koji
AU - Nazir, K. H.M.Nazmul Hussain
AU - Wada, Erina
AU - Suyama, Akiko
AU - Takegawa, Kaoru
AU - Goto, Masatoshi
AU - Nakamura, Kanji
AU - Furukawa, Kensuke
N1 - Funding Information:
We thank Towakagaku Co., Ltd., for the supply of chloroethene-contaminated soil samples. And we are grateful the Support Center for Education and Research of Kyushu University for the technical expertise of DNA sequencing analysis. This work was supported by a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2011
Y1 - 2011
N2 - A microbial consortium that reductively dechlorinates trichloroethene, cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with methanogenesis was enriched from chloroethene-contaminated soil from Japan. Dechlorination activity was maintained for over 4 years. Using quantitative polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were detected. Their growth and dechlorination activities were classified into two types: one that grows by converting cis-DCE to ethene and the other that grows by converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC reductive dehalogenases were detected. Inhibitors of methanogenesis (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of cis-DCE and of VC respectively. These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides".
AB - A microbial consortium that reductively dechlorinates trichloroethene, cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with methanogenesis was enriched from chloroethene-contaminated soil from Japan. Dechlorination activity was maintained for over 4 years. Using quantitative polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were detected. Their growth and dechlorination activities were classified into two types: one that grows by converting cis-DCE to ethene and the other that grows by converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC reductive dehalogenases were detected. Inhibitors of methanogenesis (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of cis-DCE and of VC respectively. These results suggest that methanogens and sulfate-reducing bacteria can play a significant role in dechlorination by "Dehalococcoides".
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U2 - 10.1271/bbb.110028
DO - 10.1271/bbb.110028
M3 - Article
C2 - 21737937
AN - SCOPUS:79960757192
SN - 0916-8451
VL - 75
SP - 1268
EP - 1274
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 7
ER -