TY - JOUR
T1 - Ent-trachylobane-3β-hydroperoxide, a new diterpene from the root bark of Chrozophora oblongifolia (Fam.; Euphorbiaceae)
AU - Kamel, Mohamed R.
AU - Nafady, Alaa M.
AU - Hassanein, Ahmed M.M.
AU - Hassan, Ahmed R.
AU - Shimizu, Kuniyoshi
AU - Ibrahim, Reham R.
AU - Haggag, Eman G.
N1 - Funding Information:
We would like to acknowledge the department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan, for carrying out the spectroscopic analysis and cytotoxicity study. The authors also would like to thanks to Mr. Hossam El-Feky, a pharmacist in Egyptian ambulance Authority in South Sinai for his great efforts in collecting the plant material, which is the base for this work.
Publisher Copyright:
© 2019 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - New ent-trachylobane-3β-hydroperoxide (5) together with four known compounds, ricinine (1), trimethoxy ellagic acid (2), dimethoxy ellagic acid (3) and aleurotolic acid (4) were isolated from the methylene chloride fraction of the root bark of Chrozophora oblongifolia. The structures of these secondary metabolites were elucidated by using different spectroscopic techniques, 1H NMR, 13C NMR, HSQC, HMBC, 1H-1H COSY, NOESY, HR-ESI-MS, EI-MS and comparison with published data. Ent-trachylobane-3β-hydroperoxide showed moderate cytotoxic activity by MTT assay method against human breast cancer cells (MCF-7) and human hepatocyte-derived carcinoma cells (Huh-7) with IC50 values of 24.53 and 34.13 µM, in comparison with IC50 values of 23.47 µM and 15.82 µM for 5-fluorouracil respectively.
AB - New ent-trachylobane-3β-hydroperoxide (5) together with four known compounds, ricinine (1), trimethoxy ellagic acid (2), dimethoxy ellagic acid (3) and aleurotolic acid (4) were isolated from the methylene chloride fraction of the root bark of Chrozophora oblongifolia. The structures of these secondary metabolites were elucidated by using different spectroscopic techniques, 1H NMR, 13C NMR, HSQC, HMBC, 1H-1H COSY, NOESY, HR-ESI-MS, EI-MS and comparison with published data. Ent-trachylobane-3β-hydroperoxide showed moderate cytotoxic activity by MTT assay method against human breast cancer cells (MCF-7) and human hepatocyte-derived carcinoma cells (Huh-7) with IC50 values of 24.53 and 34.13 µM, in comparison with IC50 values of 23.47 µM and 15.82 µM for 5-fluorouracil respectively.
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U2 - 10.1080/14786419.2019.1687477
DO - 10.1080/14786419.2019.1687477
M3 - Article
C2 - 31691590
AN - SCOPUS:85074849041
VL - 35
SP - 3177
EP - 3184
JO - Natural Product Research
JF - Natural Product Research
SN - 1478-6419
IS - 19
ER -
