Enterocin F4-9, a novel O-linked glycosylated bacteriocin

Mohamed Abdelfattah Maky, Naoki Ishibashi, Takeshi Zendo, Rodney Honrada Perez, Jehan Ragab Doud, Mohamed Karmi, Kenji Sonomoto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Enterococcus faecalis F4-9 isolated from Egyptian salted-fermented fish produces a novel bacteriocin, termed enterocin F4-9. Enterocin F4-9 was purified from the culture supernatant by three steps, and its molecular mass was determined to be 5,516.6 Da by mass spectrometry. Amino acid and DNA sequencing showed that the propeptide consists of 67 amino acid residues, with a leader peptide containing a double glycine cleavage site to produce a 47-amino-acid mature peptide. Enterocin F4-9 is modified by two molecules of N-acetylglucosamine β-O-linked to Ser37 and Thr46. The O-linked N-acetylglucosamine moieties are essential for the antimicrobial activity of enterocin F4-9. Further analysis of the enterocin F4-9 gene cluster identified enfC, which has high sequence similarity to a glycosyltransferase. The antimicrobial activity of enterocin F4-9 covered a limited range of bacteria, including, interestingly, a Gram-negative strain, Escherichia coli JM109. Enterocin F4-9 is sensitive to protease, active at a wide pH range, and moderately resistant to heat.

Original languageEnglish
Pages (from-to)4819-4826
Number of pages8
JournalApplied and environmental microbiology
Volume81
Issue number14
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

enterocins
Bacteriocins
bacteriocins
antimicrobial activity
amino acid
peptide
N-acetylglucosamine
Acetylglucosamine
cleavage
amino acids
mass spectrometry
anti-infective agents
DNA
fermented fish
bacterium
gene
Amino Acids
Glycosyltransferases
glycosyltransferases
fish

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Maky, M. A., Ishibashi, N., Zendo, T., Perez, R. H., Doud, J. R., Karmi, M., & Sonomoto, K. (2015). Enterocin F4-9, a novel O-linked glycosylated bacteriocin. Applied and environmental microbiology, 81(14), 4819-4826. https://doi.org/10.1128/AEM.00940-15

Enterocin F4-9, a novel O-linked glycosylated bacteriocin. / Maky, Mohamed Abdelfattah; Ishibashi, Naoki; Zendo, Takeshi; Perez, Rodney Honrada; Doud, Jehan Ragab; Karmi, Mohamed; Sonomoto, Kenji.

In: Applied and environmental microbiology, Vol. 81, No. 14, 01.01.2015, p. 4819-4826.

Research output: Contribution to journalArticle

Maky, MA, Ishibashi, N, Zendo, T, Perez, RH, Doud, JR, Karmi, M & Sonomoto, K 2015, 'Enterocin F4-9, a novel O-linked glycosylated bacteriocin', Applied and environmental microbiology, vol. 81, no. 14, pp. 4819-4826. https://doi.org/10.1128/AEM.00940-15
Maky, Mohamed Abdelfattah ; Ishibashi, Naoki ; Zendo, Takeshi ; Perez, Rodney Honrada ; Doud, Jehan Ragab ; Karmi, Mohamed ; Sonomoto, Kenji. / Enterocin F4-9, a novel O-linked glycosylated bacteriocin. In: Applied and environmental microbiology. 2015 ; Vol. 81, No. 14. pp. 4819-4826.
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