Entrainment of a circadian clock in vitro

Hiroshi Ito, Takuya Yoshida, Yoriko Murayama, Masato Nakajima, Takao Kondo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Circadian rhythms are the physiological oscillations with about 24 h periods that have period stability under various circumstances. While gene regulatory feedback loop has been proposed for the model of the origin of the circadian oscillation, my colleagues and I had proposed an alternative model. The cyanobacterial circadian clock can be reconstituted in vitro only by mixing the three clock proteins, KaiA, KaiB, and KaiC, with ATP. Namely, the ratio of phosphorylated KaiC oscillates every 24 h in the mixture. This simple biochemical reaction shows self-sustained oscillation like Belosov-Zhabotinsky reaction. Thus, KaiC phosphorylation rhythm can be core oscillator producing cyanobacterial circadian clock. In this paper, I will discuss entrainment of the "in vitro clock" by temperature cycles.

Original languageEnglish
Title of host publicationProceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10
Pages848-849
Number of pages2
Publication statusPublished - Dec 1 2010
Externally publishedYes
Event15th International Symposium on Artificial Life and Robotics, AROB '10 - Beppu, Oita, Japan
Duration: Feb 4 2010Feb 6 2010

Publication series

NameProceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10

Other

Other15th International Symposium on Artificial Life and Robotics, AROB '10
CountryJapan
CityBeppu, Oita
Period2/4/102/6/10

Fingerprint

Clocks
Phosphorylation
Adenosinetriphosphate
Genes
Proteins
Feedback
Temperature

All Science Journal Classification (ASJC) codes

  • Artificial Intelligence
  • Computer Vision and Pattern Recognition
  • Human-Computer Interaction

Cite this

Ito, H., Yoshida, T., Murayama, Y., Nakajima, M., & Kondo, T. (2010). Entrainment of a circadian clock in vitro. In Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10 (pp. 848-849). (Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10).

Entrainment of a circadian clock in vitro. / Ito, Hiroshi; Yoshida, Takuya; Murayama, Yoriko; Nakajima, Masato; Kondo, Takao.

Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10. 2010. p. 848-849 (Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ito, H, Yoshida, T, Murayama, Y, Nakajima, M & Kondo, T 2010, Entrainment of a circadian clock in vitro. in Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10. Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10, pp. 848-849, 15th International Symposium on Artificial Life and Robotics, AROB '10, Beppu, Oita, Japan, 2/4/10.
Ito H, Yoshida T, Murayama Y, Nakajima M, Kondo T. Entrainment of a circadian clock in vitro. In Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10. 2010. p. 848-849. (Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10).
Ito, Hiroshi ; Yoshida, Takuya ; Murayama, Yoriko ; Nakajima, Masato ; Kondo, Takao. / Entrainment of a circadian clock in vitro. Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10. 2010. pp. 848-849 (Proceedings of the 15th International Symposium on Artificial Life and Robotics, AROB 15th'10).
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