TY - JOUR
T1 - Entrapment of Lavandula vera cells and production of pigments by entrapped cells
AU - Nakajima, Hiroki
AU - Sonomoto, Kenji
AU - Usui, Naoki
AU - Sato, Fumihiko
AU - Yamada, Yasuyuki
AU - Tanaka, Atsuo
AU - Fukui, Saburo
N1 - Funding Information:
This work was supported in part by a grant-in-aid for research from the Ministry of Education, Science and Culture, Japan.
PY - 1985/3
Y1 - 1985/3
N2 - Finely suspended cells of Lavandula vera were obtained by cultivating the cells in the presence of 50 mM CaCl2 and entrapped homogeneously in gels formed from natural polysaccharides, agar, alginate and κ-carrageenan. The gel-entrapped cells grew well inside the gel matrices, the growth being confirmed by the increases in oxygen uptake, cell number and chlorophyll content. Blue pigments were synthesized de novo in the presence of l-cysteine by the gel-entrapped cells as well as by the free counterparts. Calcium alginate gel-entrapped cells were employed for the repeated production of the pigments for over 7 months by alternating growth and production phases. Entrapment with adequate gels stabilized markedly the viability and the pigment productivity of the plant cells.
AB - Finely suspended cells of Lavandula vera were obtained by cultivating the cells in the presence of 50 mM CaCl2 and entrapped homogeneously in gels formed from natural polysaccharides, agar, alginate and κ-carrageenan. The gel-entrapped cells grew well inside the gel matrices, the growth being confirmed by the increases in oxygen uptake, cell number and chlorophyll content. Blue pigments were synthesized de novo in the presence of l-cysteine by the gel-entrapped cells as well as by the free counterparts. Calcium alginate gel-entrapped cells were employed for the repeated production of the pigments for over 7 months by alternating growth and production phases. Entrapment with adequate gels stabilized markedly the viability and the pigment productivity of the plant cells.
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U2 - 10.1016/0168-1656(85)90046-X
DO - 10.1016/0168-1656(85)90046-X
M3 - Article
AN - SCOPUS:0000115624
SN - 0168-1656
VL - 2
SP - 107
EP - 117
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 2
ER -