Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype

Keina Monda, Juntaro Negi, Atsuhiro Iio, Kensuke Kusumi, Mikiko Kojima, Mimi Hashimoto, Hitoshi Sakakibara, Koh Iba

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The Arabidopsis Cape Verde Islands (Cvi-0) ecotype is known to differ from other ecotypes with respect to environmental stress responses. We analyzed the stomatal behavior of Cvi-0 plants, in response to environmental signals. We investigated the responses of stomatal conductance and aperture to high [CO 2] in the Cvi-0 and Col-0 ecotypes. Cvi-0 showed constitutively higher stomatal conductance and more stomatal opening than Col-0. Cvi-0 stomata opened in response to light, but the response was slow. Under low humidity, stomatal opening was increased in Cvi-0 compared to Col-0. We then assessed whether low humidity affects endogenous ABA levels in Cvi-0. In response to low humidity, Cvi-0 had much higher ABA levels than Col-0. However, epidermal peels experiments showed that Cvi-0 stomata were insensitive to ABA. Measurements of organic and inorganic ions in Cvi-0 guard cell protoplasts indicated an over-accumulation of osmoregulatory anions (malate and Cl -). This irregular anion homeostasis in the guard cells may explain the constitutive stomatal opening phenotypes of the Cvi-0 ecotype, which lacks high [CO 2]-induced and low humidity-induced stomatal closure.

Original languageEnglish
Pages (from-to)555-563
Number of pages9
JournalPlanta
Volume234
Issue number3
DOIs
Publication statusPublished - Sep 1 2011

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Ecotype
environmental law
Humidity
ecotypes
Arabidopsis
humidity
guard cells
Carbon Monoxide
stomata
anions
Anions
stomatal conductance
Cape Verde
inorganic ions
Protoplasts
malates
Islands
protoplasts
plant response
stress response

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype. / Monda, Keina; Negi, Juntaro; Iio, Atsuhiro; Kusumi, Kensuke; Kojima, Mikiko; Hashimoto, Mimi; Sakakibara, Hitoshi; Iba, Koh.

In: Planta, Vol. 234, No. 3, 01.09.2011, p. 555-563.

Research output: Contribution to journalArticle

Monda, Keina ; Negi, Juntaro ; Iio, Atsuhiro ; Kusumi, Kensuke ; Kojima, Mikiko ; Hashimoto, Mimi ; Sakakibara, Hitoshi ; Iba, Koh. / Environmental regulation of stomatal response in the Arabidopsis Cvi-0 ecotype. In: Planta. 2011 ; Vol. 234, No. 3. pp. 555-563.
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