Environmental stresses activate a tomato SNF1-related protein kinase 2 homolog, SlSnRK2C

Takashi Yuasa, Yoko Tomikubo, Takashi Yamauchi, Akira Inoue, Mari Iwaya-Inoue

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We isolated an SNF1-related kinase 2 homolog (SlSnRK2C) from tomato (Solanum lycopersicum L. cv. Micro-Tom) which has significant similarlity to AtSRK2C (72% amino acid identity). We profiled expression levels and biochemical properties of SlSnRK2C. In contrast to AtSRK2C which phosphorylates histone H1 and is mainly expressed in root chip, SlSnRK2C phosphorylates myelin basic protein but not histone H1 and SlSnRK2C mRNA is expressed in leaves, flowers and fruits but not in roots. Immunoblot by anti-SlSnRK2C-COOH terminus specific antiserum shows that SlSnRK2C is expressed remarkably in developing fruits, and weakly in flowers, mature green fruits and shoots, but marginally in mature red fruits or roots. SlSnRK2C is stimulated strongly by chilling and NaCl stress. These observations suggests that SlSnRK2C is possibly involved in fruit development and stress signaling in response to salt and chiling stress.

Original languageEnglish
Pages (from-to)401-408
Number of pages8
JournalPlant Biotechnology
Volume24
Issue number4
DOIs
Publication statusPublished - Jan 1 2007

Fingerprint

Lycopersicon esculentum
protein kinases
Fruit
tomatoes
fruits
histones
Histones
flowers
Solanum lycopersicum
Myelin Basic Protein
antiserum
fruiting
phosphotransferases (kinases)
Immune Sera
salts
Salts
amino acids
shoots
SNF1-related protein kinases
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Environmental stresses activate a tomato SNF1-related protein kinase 2 homolog, SlSnRK2C. / Yuasa, Takashi; Tomikubo, Yoko; Yamauchi, Takashi; Inoue, Akira; Iwaya-Inoue, Mari.

In: Plant Biotechnology, Vol. 24, No. 4, 01.01.2007, p. 401-408.

Research output: Contribution to journalArticle

Yuasa, Takashi ; Tomikubo, Yoko ; Yamauchi, Takashi ; Inoue, Akira ; Iwaya-Inoue, Mari. / Environmental stresses activate a tomato SNF1-related protein kinase 2 homolog, SlSnRK2C. In: Plant Biotechnology. 2007 ; Vol. 24, No. 4. pp. 401-408.
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