Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp

Eisaku Hokazono, Hideto Tamezane, Taeko Hotta, Yuzo Kayamori, Susumu Osawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Background: In human serum, as for phospholipids not containing choline, phosphatidylethanolamine (PE) exists approximately 5% in a whole phospholipid. PE is well known as one of the main components of biological membranes, and also plays important roles that contribute to apoptosis and cell signaling. However, it could not measure PE with other phospholipids due to a lack of choline in them. Methods: Using an amine oxidase (EC 1.4.3.6), from Arthrobacter species, a simple and rapid enzymatic assay for measurements of PE in serum was established. That assay used the Hitachi 7170 analyzer to evaluate the analytical performance. Results: The average within-run CVs were 0.38-1.27% (n=20) at 69-160μmol/l. The correlation between values obtained with the present method (y) and the high-performance liquid chromatography (HPLC) method (x) was: y=0.944x+9.441 (r=0.977, Sy|x=5.82, n=34). In addition, the reference interval of healthy subjects was 115±45μmol/l. Conclusions: This new enzymatic method shows a high specificity for serum PE and can be easily applied to an automated analyzer. The present method is available as a novel marker of changes in the clinical condition of serum phospholipids.

Original languageEnglish
Pages (from-to)1436-1440
Number of pages5
JournalClinica Chimica Acta
Volume412
Issue number15-16
DOIs
Publication statusPublished - Jul 15 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Enzymatic assay of phosphatidylethanolamine in serum using amine oxidase from Arthrobacter sp'. Together they form a unique fingerprint.

Cite this