Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system

Tatsuo Maruyama, Jun Ichi Uchida, Tomohiro Ohkawa, Toru Futami, Koji Katayama, Kei Ichiro Nishizawa, Ken Ichiro Sotowa, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

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Abstract

Enzymatic degradation of p-chlorophenol was carried out in a two-phase flow in a microchannel (100 μm width, 25 μm depth) fabricated on a glass plate (70 mm × 38 mm). This is the first report on the enzymatic reaction in a two-phase flow on a microfluidic device. The surface of the microchannel was partially modified with octadecylsilane groups to be hydrophobic, thus allowing clear phase separation at the end-junction of the microchannel. The enzyme (laccase), which is surface active, was solubilized in a succinic aqueous buffer and the substrate (p-chlorophenol) was in isooctane. The degradation of p-chlorophenol occurred mainly at the aqueous-organic interface in the microchannel. We investigated the effects of flow velocity and microchannel shape on the enzymatic degradation of p-chlorophenol. Assuming that diffusion of the substrate (p-chlorophenol) is the rate-limiting step in the enzymatic degradation of p-chlorophenol in the microchannel, we proposed a simple theoretical model for the degradation in the microchannel. The calculated degradation values agreed well with the experimental data.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalLab on a Chip
Volume3
Issue number4
DOIs
Publication statusPublished - Nov 2003

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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    Maruyama, T., Uchida, J. I., Ohkawa, T., Futami, T., Katayama, K., Nishizawa, K. I., Sotowa, K. I., Kubota, F., Kamiya, N., & Goto, M. (2003). Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. Lab on a Chip, 3(4), 308-312. https://doi.org/10.1039/b309982b