Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system

Tatsuo Maruyama, Jun Ichi Uchida, Tomohiro Ohkawa, Toru Futami, Koji Katayama, Kei Ichiro Nishizawa, Ken Ichiro Sotowa, Fukiko Kubota, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Enzymatic degradation of p-chlorophenol was carried out in a two-phase flow in a microchannel (100 μm width, 25 μm depth) fabricated on a glass plate (70 mm × 38 mm). This is the first report on the enzymatic reaction in a two-phase flow on a microfluidic device. The surface of the microchannel was partially modified with octadecylsilane groups to be hydrophobic, thus allowing clear phase separation at the end-junction of the microchannel. The enzyme (laccase), which is surface active, was solubilized in a succinic aqueous buffer and the substrate (p-chlorophenol) was in isooctane. The degradation of p-chlorophenol occurred mainly at the aqueous-organic interface in the microchannel. We investigated the effects of flow velocity and microchannel shape on the enzymatic degradation of p-chlorophenol. Assuming that diffusion of the substrate (p-chlorophenol) is the rate-limiting step in the enzymatic degradation of p-chlorophenol in the microchannel, we proposed a simple theoretical model for the degradation in the microchannel. The calculated degradation values agreed well with the experimental data.

Original languageEnglish
Pages (from-to)308-312
Number of pages5
JournalLab on a Chip
Volume3
Issue number4
DOIs
Publication statusPublished - Jan 1 2003

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Microchannels
Two phase flow
Degradation
Lab-On-A-Chip Devices
Laccase
Succinic Acid
Substrates
Glass
4-chlorophenol
Microfluidics
Buffers
Flow velocity
Phase separation
Theoretical Models
Enzymes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Maruyama, T., Uchida, J. I., Ohkawa, T., Futami, T., Katayama, K., Nishizawa, K. I., ... Goto, M. (2003). Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. Lab on a Chip, 3(4), 308-312. https://doi.org/10.1039/b309982b

Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. / Maruyama, Tatsuo; Uchida, Jun Ichi; Ohkawa, Tomohiro; Futami, Toru; Katayama, Koji; Nishizawa, Kei Ichiro; Sotowa, Ken Ichiro; Kubota, Fukiko; Kamiya, Noriho; Goto, Masahiro.

In: Lab on a Chip, Vol. 3, No. 4, 01.01.2003, p. 308-312.

Research output: Contribution to journalArticle

Maruyama, T, Uchida, JI, Ohkawa, T, Futami, T, Katayama, K, Nishizawa, KI, Sotowa, KI, Kubota, F, Kamiya, N & Goto, M 2003, 'Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system', Lab on a Chip, vol. 3, no. 4, pp. 308-312. https://doi.org/10.1039/b309982b
Maruyama T, Uchida JI, Ohkawa T, Futami T, Katayama K, Nishizawa KI et al. Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. Lab on a Chip. 2003 Jan 1;3(4):308-312. https://doi.org/10.1039/b309982b
Maruyama, Tatsuo ; Uchida, Jun Ichi ; Ohkawa, Tomohiro ; Futami, Toru ; Katayama, Koji ; Nishizawa, Kei Ichiro ; Sotowa, Ken Ichiro ; Kubota, Fukiko ; Kamiya, Noriho ; Goto, Masahiro. / Enzymatic degradation of p-chlorophenol in a two-phase flow microchannel system. In: Lab on a Chip. 2003 ; Vol. 3, No. 4. pp. 308-312.
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